Description

The ChromaLink Biotin, maleimide version reacts with thiols groups on proteins. Soluble in DMF. Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. Highlights:

* Fast and easy method to biotinylate and determine the amount of labeling.
* Label and determine the extent of biotin labeling with one reagent.
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample.
* Five aliquots, able to label up to 10 milligrams of protein per aliquot.

1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes. steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications
3. A maleimide group, known to efficiently couple to thiols.

Applications include:

* Biotinylate protein or peptides
* Quantitate the extent of biotin labeling
* Optimize biotin-avidin complexes

Home

Services

Molecular Biology

Proteins & Peptides

Antibodies

ELISA Kits

Chemicals

Cell Biology

Contact

My Syd Labs

Email Notifications

Maleimide Biotin Reagent

Note

Note

Order Offline