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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: PS/2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse/human VLA-4 (CD49d) monoclonal antibody (clone: PS/2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: PS/2) specifically binds to mouse/human VLA-4 (CD49d).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human VLA-4 (CD49d) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse/human VLA-4 (CD49d) monoclonal antibody of clone PS/2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Th17 lymphocytes traffic to the central nervous system independently of ?4 integrin expression during EAE.
Rothhammer, V., et al. J Exp Med. 2011 Nov 21;208(12):2465-76. doi: 10.1084/jem.20110434. PMID: 22025301
The integrin ?4?1 (VLA-4) is used by encephalitogenic T cells to enter the central nervous system (CNS). ...Early studies in adoptive transfer experimental autoimmune encephalomyelitis (EAE) in mice suggested that only VLA-4?expressing CNS antigen-specific T cell lines were able to induce EAE, and blockade of VLA-4 by antibodies to ?4 abrogated their encephalitogenic potential. ...In the present study, we investigated the requirement for ?4 integrins, particularly VLA-4 (?4?1, CD49d/CD29), and also the ?2 integrin LFA-1 (?L?2, CD11a/CD18) in the entry of T cell subsets into the CNS. ...Before transfer, Th1 and Th17 cells were incubated with control immunoglobulin or blocking antibodies to ?4 integrin (PS/2) and after transfer, host mice were treated with either control IgG or antibodies to ?4 integrin until the development of first signs of disease. ...Animals were thereafter treated with i.p. PS/2 or 200-?g isotype control antibody injections every 3 d until the development of clinical disease.
Tags: anti-mouse VLA-4 (CD49d) PS/2; anti-mouse VLA-4 (CD49d) PS/2 antibody
Analysis of behavior and trafficking of dendritic cells within the brain during toxoplasmic encephalitis.
John, B., et al. PLoS Pathog. 2011 Sep;7(9):e1002246. doi: 10.1371/journal.ppat.1002246. PMID: 21949652
Signaling downstream of the G?i coupled receptors has been shown to increase the affinity of many integrins particularly LFA-1 and VLA-4, for their respective ligands. ...Blocking VLA-4 had minimal effect on the migration of DCs into the brain using this adoptive transfer system. ...Recently the integrin VLA-4 has been shown to be involved in the recruitment of immature DCs into the CNS during EAE. ...These clinical studies indicate the importance of LFA-1 in addition to VLA-4 in the migration of immune effectors into the CNS. ...To block LFA-1 or VLA-4 in vitro, the purified DCs were treated with 30 ?g/ml of anti CD11a antibody (Clone M-17/4, BioXcell, West Lebanon, NH) or anti VLA-4 (Clone PS/2, BioXcell, West Lebanon, NH) for 3 hours prior to transfer.
Tags: anti-mouse VLA-4 (CD49d) PS/2 antibody in cancer research; anti-mouse VLA-4 (CD49d) PS/2 in mouse tumor model
For more references about Anti-mouse/human VLA-4 (CD49d) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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