Anti-Mouse / Human CD44 Monoclonal Antibodies

PA007622.r2b: In Vivo Grade Recombinant Anti-mouse/human CD44 Monoclonal Antibody (Clone: IM7.8.1), Rat IgG2b Kappa

Recombinant rat IgG2b Monoclonal Antibody.
Clone: IM7.8.1.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse/human CD44 monoclonal antibody (clone: IM7.8.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: IM7.8.1) specifically binds to mouse/human CD44.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human CD44 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse/human CD44 monoclonal antibody of clone IM7.8.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse/human CD44 antibody (Clone: IM7.8.1):

1、CD44-mediated hematopoietic progenitor cell adhesion and its complex role in myelopoiesis.
Hidalgo, A., et al. J Hematother Stem Cell Res. 2002 Jun;11(3):539-47. doi: 10.1089/15258160260091004. PMID: 12183839
Although MA-8 and another anti-CD44 antibody, IM7.8.1 (anti-mouse CD44 IM7.8.1), blocked adhesion of hematopoietic cells to hyaluronate, adhesion to BM stroma was notably increased by these mAb, suggesting minor roles for CD44/hyaluronate interaction and the triggering of additional adhesion pathways upon CD44 engagement. ...Instead, IM7.8.1 (anti-mouse CD44 IM7.8.1 mAb) completely blocked myelopoiesis, whereas an anti-α4 integrin mAb increased CFU-GM amounts. ...During hematopoiesis in the bone marrow (BM), hematopoietic progenitor cells (HPC) proliferate and differentiate in intimate contact with BM stromal cells in a highly regulated interplay among cytokines, chemokines, and adhesion molecules. ...Using the mouse BM stromal cell line MS-5 as immunogen, we raised monoclonal antibodies (mAb) against surface proteins that could participate in interactions between HPC and stroma. ...Flow cytometry and immunodepletion analysis indicated that two antibodies, MA-8 and MA-2, recognized CD44.
Tags: anti-mouse CD44 IM7.8.1; anti-mouse CD44 IM7.8.1 mAb

2、CD44 and hyaluronic acid regulate in vivo iNOS expression and metalloproteinase activity in murine air-pouch inflammation.
Cabrera, P. V., et al. Inflamm Res. 2004 Oct;53(10):556-66. doi: 10.1007/s00011-004-1295-8. PMID: 15597151
Leukocytes were obtained from zymosan-air pouches after the intra-pouch injection of anti-CD44 IM7.8.1 (anti-mouse CD44 IM7.8.1 in vivo), isotype control, HMW-HA, LMW-HA or PBS. ...IM7.8.1 decreased iNOS mRNA levels and the activity of both MMP-9 and MMP-2 eight h after injection into zymosan air pouch while IM7.8.1 (anti-mouse CD44 IM7.8.1 in animal model), HMW-HA and LMW-HA had no effect on IL1-β or TNF-α mRNA levels. ...IM7.8.1 had consistent anti-inflammatory effects, reducing iNOS, MMP-9 and MMP-2. ...To evaluate the effects of anti-CD44 IM7.8.1 antibody, HMW-HA and LMW-HA on leukocyte migration and adhesion, and the induction of proinflammatory mediators, in mouse air-pouch inflammation induced by zymosan. ...Both CD44 and HA can modulate leukocyte migration and induction of proinflammatory mediators in mouse zymosan air pouch inflammation.
Tags: anti-mouse CD44 IM7.8.1 in vivo; anti-mouse CD44 IM7.8.1 in animal model

3、CD44 involvement in experimental collagen-induced arthritis (CIA).
Nedvetzki, S., et al. J Autoimmun. 1999 Aug;13(1):39-47. doi: 10.1006/jaut.1999.0294. PMID: 10441166
Even accelerated disease developed after two injections of type II collagen was markedly inhibited by IM7.8.1 anti-CD44 mAb (anti-mouse CD44 IM7.8.1 antibody in cancer research). ...Although KM81 anti-CD44 mAb is a less efficient anti-arthritogenic reagent than IM7.8.1, its Fab' fragments partially inhibit CIA. ...This finding implies that the antibody blocks CD44 function rather than modulating CD44 cell surface expression or mediating Fc-dependent activities. ...Histopathological analysis revealed that the anti-CD44 mAb markedly reduces the synovial inflammatory cellular response and the consequent damage to the joint. ...Therefore, CD44 targeting with specific monoclonal antibodies (mAbs) should be useful for the inhibition of collagen-induced arthritis (CIA) as well as other autoimmune diseases that are dependent on inflammatory cells.
Tags: anti-mouse CD44 IM7.8.1 antibody in cancer research; anti-mouse CD44 IM7.8.1 in mouse tumor model

4、CD44 is involved in selective leucocyte extravasation during inflammatory central nervous system disease.
Brennan, F. R., et al. Immunology. 1999 Nov;98(3):427-35. doi: 10.1046/j.1365-2567.1999.00894.x. PMID: 10583604
However when a CD44-specific mAb IM7.8.1 (IM7) (anti-mouse CD44 IM7.8.1 antibody of low endotoxin) was administered to mice, it inhibited lymphocyte infiltration into a cutaneous hypersensitivity site and arthritic joints, whilst normal leucocyte migration into lymphoid tissue was unaffected. ...Cells were incubated for 40 min at 4° with mAbs specific for l-selectin (CD62L; clone MEL-14; ATCC), VLA-4 (α4 integrin/CD49d; clone R1/2; ATCC), CD44s (clone IM7.8.1 (anti-mouse CD44 (IM7.8.1) in vivo antibody); ATCC) or CD45RB (clone 16a), and detected with fluorescein isothiocyanate (FITC)-conjugated rabbit anti-rat immunoglobulin (Serotec Ltd, Kidlington, Oxford, UK). ...Indeed, administration of a CD44-specific antibody (IM7.8.1) induced leucocyte CD44 shedding and both prevented the development and ameliorated the severity of established EAE by inhibiting mononuclear cell infiltration into the CNS. ...This study contests previous reports that dismissed a role for CD44 in inflammation of the CNS and, coupled with observations in murine dermatitis and arthritis, suggests that CD44 is involved in the homing of primed lymphocytes to sites of inflammation. ...Single-cell suspensions were prepared from inguinal lymph nodes and peripheral blood.
Tags: anti-mouse CD44 IM7.8.1 antibody of low endotoxin; anti-mouse CD44 (IM7.8.1) in vivo antibody

For more references about anti-mouse/human CD44 antibody (Clone: IM7.8.1) please contact our scientific support team with message@sydlabs.com.