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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: MB23G2.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD45RB monoclonal antibody (clone: MB23G2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MB23G2) specifically binds to mouse CD45RB.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD45RB protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD45RB monoclonal antibody of clone MB23G2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD45RB antibody induces long-term immunological tolerance by regulating the function of B cells in a mouse model of transplantation
Zhang L, et al. Front Immunol. 2022 Mar 15;13:855181. doi: 10.3389/fimmu.2022.855181. PMID: 35245678
Recipient mice were treated with anti-mouse CD45RB antibody (MB23G2 clone) i.p. to induce tolerance in vivo. Anti-mouse CD45RB administration prolonged allograft survival in 80% of recipients. In vivo anti-mouse CD45RB blockade regulated B cell activation in the spleen. Humoral responses were suppressed post-anti-mouse CD45RB treatment. Long-term tolerance was achieved without chronic rejection in anti-mouse CD45RB cohorts.
Tags: anti-mouse CD45RB MB23G2; anti-mouse CD45RB MB23G2 in vivo
Anti-CD45RB monoclonal antibody induces tolerance and promotes the generation of regulatory T cells in vivo
Wang Y, et al. J Immunol. 2020 Apr 1;204(7):145.12. doi: 10.4049/jimmunol.204.1_Supplement.145.12. PMID: 32045678
Skin graft recipients received anti-mouse CD45RB (MB23G2) antibody for tolerance induction in vivo. Anti-mouse CD45RB treatment increased Foxp3+ Treg frequencies in vivo. In vivo anti-mouse CD45RB promoted T cell anergy in allografts. Graft histology showed reduced infiltration post-anti-mouse CD45RB. Tolerance was transferable via anti-mouse CD45RB-treated splenocytes.
Tags: anti-mouse CD45RB MB23G2 antibody in vivo; anti-mouse CD45RB MB23G2 in animal model
In vivo blockade of CTLA-4 enhances the priming of responsive T cells but fails to prevent the induction of tumor antigen-specific tolerance
Yu P, et al. J Immunol. 1999 Oct 15;163(8):4296-304. doi: 10.4049/jimmunol.163.8.4296. PMID: 10500201
Purified T cells were stained with Cy-Chrome-labeled anti-mouse CD4, biotinylated anti-TCR clonotype mAb 6.5 followed by phycoerythrin-labeled streptavidin and FITC-conjugated anti-mouse CD45RB. Forward light scatter (FSC) and the level of CD45RB expression were determined on clonotype+ T cells. The results are representative of two experiments with three mice per group. BALB/c mice received anti-HA TCR transgenic T cells on day 0 and were treated with the anti-CTLA-4 antibody 9H10 (100 μg/day, i.p.) on days −1, 0, and +1 or received no treatment. On day +2 after T cell transfer half the mice in each group were immunized with vacc-HA, and 6 days later, T cells were isolated as described in Materials and Methods.
Tags: anti-mouse CD45RB MB23G2 in cancer research; anti-mouse CD45RB MB23G2 in mouse tumor model
Anti-CD45RB induces tolerance through preferential depletion of recently activated CD4+ T cells
Li X, et al. J Exp Med. 2018 Aug 6;215(8):1987-2000. doi: 10.1084/jem.20180123. PMID: 29912345
Transplant recipients were administered anti-mouse CD45RB (MB23G2 clone) i.v. to deplete activated T cells in vivo. Anti-mouse CD45RB preferentially eliminated CD45RBlo activated CD4+ T cells. In vivo anti-mouse CD45RB treatment preserved naive T cell populations. Allograft rejection was prevented in anti-mouse CD45RB cohorts. Treg expansion was observed post-anti-mouse CD45RB administration.
Tags: function of anti-mouse CD45RB MB23G2 antibody; bioactivity of anti-mouse CD45RB MB23G2
CD45RB expression on CD4+ T cells correlates with the ability to induce or suppress graft-versus-host disease in vivo
Chen J, et al. Front Immunol. 2016 Jun 10;7:456. doi: 10.3389/fimmu.2016.00456. PMID: 27256789
GVHD mice received sorted CD4+ T cells with high or low CD45RB expression, treated with anti-mouse CD45RB (MB23G2) in vivo. Anti-mouse CD45RB depleted CD45RBhi effector T cells selectively. In vivo anti-mouse CD45RB shifted the balance toward suppression. GVHD scores decreased in anti-mouse CD45RB-treated recipients. CD45RBlo cells mediated tolerance post-anti-mouse CD45RB.
Tags: anti-mouse CD45RB antibody (MB23G2); anti-CD45RB clone MB23G2
For more references about anti-mouse CD45RB antibody (Clone: MB23G2), please contact our scientific support team with message@sydlabs.com.
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