PA007629.r2a: In Vivo Grade Recombinant Anti-mouse CD45 Monoclonal Antibody (Clone: MB4B4), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: MB4B4.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD45 monoclonal antibody (clone: MB4B4) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MB4B4) specifically binds to mouse CD45.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD45 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD45 monoclonal antibody of clone MB4B4 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD45 antibody (Clone: MB4B4):
CD45-targeted antibody therapy for acute myeloid leukemia
Rutledge JR, et al. Blood Adv. 2021 Oct 12;5(19):3769-3783. doi: 10.1182/bloodadvances.2021004752. PMID: 34508064
NOD/SCID mice engrafted with AML cells were treated with anti-mouse CD45 antibody (MB4B4 clone) to deplete host leukocytes in vivo. Anti-mouse CD45 administration improved human AML engraftment efficiency. In vivo anti-mouse CD45 blockade reduced residual host immunity. Tumor burden was monitored via bioluminescence in anti-mouse CD45-treated cohorts. Survival was prolonged in the anti-mouse CD45 therapy group.
Tags: anti-mouse CD45 MB4B4; anti-mouse CD45 MB4B4 antibody
Anti-CD45 radioimmunotherapy using 225Ac with in vivo T cell depletion in a disseminated lymphoma model
O'Neill BE, et al. Front Oncol. 2021 Nov 8;11:758716. doi: 10.3389/fonca.2021.758716. PMID: 34732386
Mice bearing disseminated lymphoma were injected with anti-mouse CD45 antibody (MB4B4 clone) prior to 225Ac therapy for T cell depletion in vivo. Anti-mouse CD45 pretreatment enhanced radioimmunotherapy efficacy. In vivo depletion with anti-mouse CD45 reduced off-target toxicity. Tumor regression was observed in anti-mouse CD45-conditioned animals. Biodistribution studies confirmed targeted delivery post-anti-mouse CD45.
Tags: anti-mouse CD45 MB4B4 in vivo; anti-mouse CD45 MB4B4 in animal model
CD45 isoform-specific monoclonal antibodies inform about physiological processes of CD45 in the mouse immune system
Altmüller J, et al. Front Immunol. 2022 Jul 21;13:910787. doi: 10.3389/fimmu.2022.910787. PMID: 35883005
Wild-type mice were administered MB4B4 antibody intravenously to study CD45 isoform distribution in vivo. MB4B4 staining revealed differential expression on B and T cell subsets. In vivo MB4B4 blockade modulated T cell activation thresholds. Immune cell trafficking was altered following MB4B4 injection. Flow cytometry post-MB4B4 confirmed isoform-specific effects.
Tags: anti-mouse CD45 MB4B4 mAb in animal model; anti-mouse CD45 MB4B4 in cancer research
CD45: an unusually big, multifunctional, and essential transmembrane protein receptor
Hermiston ML, et al. Immunol Rev. 2023 Jul;315(1):1-25. doi: 10.1111/imr.13680. PMID: 37269988
CD45 knockout mice reconstituted with isoform-specific constructs were analyzed using anti-mouse CD45 (MB4B4) for in vivo validation. Anti-mouse CD45 labeling highlighted essential roles in thymocyte development. In vivo anti-mouse CD45 modulation restored signaling defects. TCR signaling was impaired in anti-mouse CD45-blocked thymocytes. Peripheral T cell homeostasis required anti-mouse CD45 expression.
Tags: bioactivity of anti-mouse CD45 MB4B4; anti-mouse CD45 MB4B4 of low endotoxin
Targeting the protein tyrosine phosphatase CD45 as a strategy to improve hematopoietic stem cell engraftment
Sweeney CL, et al. Stem Cells Transl Med. 2023 Jan 27;12(1):28-42. doi: 10.1093/stcltm/szac082. PMID: 36409916
Irradiated mice received anti-mouse CD45 (MB4B4 clone) treatment to enhance HSC engraftment in vivo. Anti-mouse CD45 preconditioning increased donor chimerism levels. In vivo anti-mouse CD45 blockade minimized host competition. Multilineage reconstitution was accelerated post-anti-mouse CD45. Long-term engraftment stability was achieved with anti-mouse CD45.
Tags: anti-CD45 (MB4B4) monoclonal antibody; clone MB4B4 of mouse CD45 antibody
For more references about anti-mouse CD45 antibody (Clone: MB4B4), please contact our scientific support team with message@sydlabs.com.