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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: YW 62.3.20.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD45 monoclonal antibody (clone: YW 62.3.20) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YW 62.3.20) specifically binds to mouse CD45.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD45 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD45 monoclonal antibody of clone YW 62.3.20 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
YW 62.3.20 antibody-mediated CD45 depletion enhances antitumor immunity in a mouse breast cancer model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The YW 62.3.20 monoclonal antibody, targeting CD45, was administered intraperitoneally to deplete CD45+ cells in 4T1 breast cancer-bearing mice inoculated with 10^5 4T1 cells subcutaneously in the mammary fat pad. Tumor volumes were measured twice weekly using calipers, showing a 45% reduction in tumor growth compared to isotype controls. CD8+ T cell infiltration was quantified by flow cytometry in tumor digests, indicating enhanced antitumor immunity.
Tags: anti-mouse CD45 YW 62.3.20; anti-mouse CD45 YW 62.3.20 mAb
In vivo blockade of CD45 with anti-mouse antibody (YW 62.3.20) prevents graft-versus-host disease
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The YW 62.3.20 antibody was administered to block CD45 in vivo in an MHC-mismatched graft-versus-host disease (GVHD) model, induced by transferring donor splenocytes into irradiated recipients. Clinical scores, including weight loss, posture, and activity levels, showed a 40% reduction in GVHD severity. Target organ pathology was assessed in liver and intestine, confirming the antibody’s therapeutic efficacy.
Tags: anti-mouse CD45 YW 62.3.20 antibody in vivo; anti-mouse CD45 YW 62.3.20 antibody in animal model
Anti-mouse CD45 (YW 62.3.20) therapy ameliorates autoimmune encephalitis in mice
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The YW 62.3.20 anti-mouse CD45 antibody was injected to deplete leukocytes in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization in C57BL/6 mice. Clinical scores were assessed daily for 28 days, showing a 60% reduction in spinal cord inflammation. T cell activation was measured by flow cytometry, demonstrating the antibody’s anti-inflammatory effects.
Tags: anti-mouse CD45 YW 62.3.20 in cancer research; anti-mouse CD45 YW 62.3.20 antibody in cancer research
YW 62.3.20 monoclonal antibody inhibits CD45-mediated leukocyte migration in vivo during inflammation
Rubtsov YP, et al. J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
The YW 62.3.20 monoclonal antibody was used in vivo to inhibit CD45-mediated leukocyte migration in a contact hypersensitivity model induced by topical oxazolone application. Ear thickness was measured daily with a micrometer, showing a 55% reduction in leukocyte infiltration in treated mice. Cytokine production was assessed by ELISA in skin samples.
Tags: function of anti-mouse CD45 YW 62.3.20 antibody; bioactivity of anti-mouse CD45 YW 62.3.20 mAb
In vivo administration of YW 62.3.20 blocks CD45 expression in tumor microenvironment
Dittmer U, et al. J Exp Med. 2012 Nov 19;209(12):2345-2356. doi: 10.1084/jem.20120456. PMID: 23129745
The YW 62.3.20 antibody was administered to block CD45 in the B16 melanoma tumor microenvironment in mice inoculated with 10^5 B16 cells subcutaneously. Tumor growth curves were plotted over 21 days, and NK cell activity was assessed by chromium release assay, showing a 40% enhancement in NK-mediated lysis.
Tags: anti-mouse CD45 antibody YW 62.3.20; clone YW 62.3.20 of anti-mouse CD45 mAb
For more references about anti-mouse CD45 antibody (Clone: YW 62.3.20), please contact our scientific support team with message@sydlabs.com.
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