My Cart [ 0 ]
Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD20 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD20 Monoclonal Antibodies for Flow Cytometry

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, CD20

Catalog No. Product Name Size List Price (US$) Quantity
PA007466.m2a Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa 100 ug 100.00
PA007466.m2a Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa 500 ug 200.00
PA007466.h1Fs Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa 100 ug 200.00
PA007466.h1Fs Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa 500 ug 400.00
PA007747.h1Fs-b Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa 100 ug 150.00
PA007747.h1Fs-b Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa 500 ug 300.00
Description

PA007466.m2a: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa

Recombinant  Mouse IgG2a Monoclonal Antibody. 
Clone: B9E9.
Isotype:  Mouse IgG2a Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: B9E9) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B9E9 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007466.h1Fs: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: B9E9.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: B9E9) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B9E9 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007747.h1Fs-b: Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 monoclonal antibody with site-directed  biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: B9E9.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD20 monoclonal antibody (clone: B9E9) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant B9E9 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The B9E9 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human CD20 Monoclonal Antibody (Clone: B9E9)

Savage, P., et al. Br J Cancer. 2002 Apr 22;86(8):1336-42. doi: 10.1038/sj.bjc.6600223. PMID: 11953895
The B9E9 scFvSA fusion protein, containing the single-chain variable region of the murine IgG2a anti-CD20 antibody B9E9 fused to streptavidin, was used to deliver HLA-A2/class I peptide complexes to HLA class I-negative Daudi cells. PBMCs were incubated with B9E9 scFvSA (10 ?g/ml) for 1 hour, and flow cytometry with antibodies like anti-MHC class I (W6/32), anti-CD19, anti-CD80, and anti-CD86 confirmed binding and CTL induction.
(Tags: B9E9 mAb for animal model; B9E9 mAb for flow cytometry)
Schultz, J., et al. Cancer Res. 2000 Dec 1;60(23):6663-9. PMID: 11118050
The B9E9 scFvSA fusion protein, derived from the CD20-specific murine IgG2a monoclonal antibody B9E9, was genetically engineered for pretargeted radioimmunotherapy. The V H and V L DNA fragments were amplified from B9E9 hybridoma cells, and the resulting scFvSA fusion protein showed nanomolar affinity to CD20-positive Ramos cells, comparable to the original B9E9 monoclonal antibody, as confirmed by in vitro and in vivo characterization.
(Tags: B9E9 mAb; B9E9 monoclonal antibody)
For more technical references or data sheets regarding the Anti-Human CD20 Monoclonal Antibody (Clone: B9E9) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa
Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD20 monoclonal antibodies:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)
Recombinant Anti-human CD20 monoclonal antibody (Clone: 1F5)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD20 monoclonal antibodies:
Recombinant Anti-mouse CD20 monoclonal antibody (Clone: 2H7)

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7) for flow cytometry
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9) for flow cytometry

Related Links

See our Privacy Policy