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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: B9E9.
Isotype: Mouse IgG2a Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: B9E9) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B9E9 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: B9E9.
Isotype: Human IgG1 kappa.
Source: The anti-human CD20 monoclonal antibody (clone: B9E9) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B9E9 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The B9E9 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Additional expression of T-cell engager in clinically tested oncolytic adeno-immunotherapy redirects tumor-infiltrated, irrelevant T cells against cancer cells to enhance antitumor immunity
Morita, D., et al. (2024). J Immunother Cancer. 2024 Dec 9;12(12):e009741. doi: 10.1136/jitc-2024-009741. PMID: 39653552
The study utilized fluorochrome-conjugated monoclonal antibodies, including anti-human CD20 (clone B9E9), to stain human cells and analyze them via flow cytometry using Gallios or BD FACSymphony cytometers. Eight weeks post-injection in humanized mice, PBMCs were stained with anti-human CD45 to confirm humanization. Other antibodies included anti-CD3 (UCHT1), CD4 (SK3), CD8 (RPA-T8), and multiple others to assess immune cell profiles, demonstrating enhanced antitumor immunity through T-cell redirection.
Tags: activity of B9E9 antibody; function of B9E9 antibody
WT1 Expression Levels Combined with Flow Cytometry Blast Counts for Risk Stratification of Acute Myeloid Leukemia and Myelodysplastic Syndromes
Giudice, V., et al. (2021). Biomedicines. 2021 Apr 6;9(4):387. doi: 10.3390/biomedicines9040387. PMID: 33917307
For immunophenotyping, 50 µL of fresh heparinized peripheral blood (PB) or bone marrow (BM) was stained with antibodies including anti-CD19 (anti-human CD20, clone B9E9) for PB and BM analysis. Samples were acquired on Navios or Navios/EX cytometers, with cell populations identified based on forward scatter and CD45 expression. The study used a panel of antibodies, including CD3, CD7, CD5, CD34, and others, to gate lymphocytes, monocytes, granulocytes, and immature cells for risk stratification in AML and MDS.
Tags: B9E9 antibody; B9E9 antibody for animal model
De novo diffuse large B-cell lymphoma with a CD20 immunohistochemistry-positive and flow cytometry-negative phenotype: molecular mechanisms and correlation with rituximab sensitivity
Tokunaga, T., et al. (2014). Cancer Sci. 2014 Jan;105(1):35-43. doi: 10.1111/cas.12307. PMID: 24147568
CD20 expression in de novo diffuse large B-cell lymphoma was confirmed using the mouse anti-CD20 antibody B9E9 (anti-human CD20, IgG2a, recognizing the B1 epitope) via flow cytometry with a BD FACSAria III. The mean fluorescence intensity (MFI) of CD20 IHC(+)/FCM(−) cells was significantly lower than IHC(+)/FCM(+) cells when stained with B9E9 or B1 antibodies. CD19 positivity confirmed B-cell lymphoma populations, and CD20 IHC was performed with the L26 antibody.
Tags: B9E9 antibody for flow cytometry; B9E9 antibody for mouse tumor model
Induction of viral and tumour specific CTL responses using antibody targeted HLA class I peptide complexes
Savage, P., et al. (2002). Br J Cancer. 2002 Apr 22;86(8):1336-42. doi: 10.1038/sj.bjc.6600223. PMID: 11953895
The B9E9 scFvSA fusion protein, containing the single-chain variable region of the murine IgG2a anti-CD20 antibody B9E9 fused to streptavidin, was used to deliver HLA-A2/class I peptide complexes to HLA class I-negative Daudi cells. PBMCs were incubated with B9E9 scFvSA (10 μg/ml) for 1 hour, and flow cytometry with antibodies like anti-MHC class I (W6/32), anti-CD19, anti-CD80, and anti-CD86 confirmed binding and CTL induction.
Tags: B9E9 mAb for animal model; B9E9 mAb for flow cytometry
A tetravalent single-chain antibody-streptavidin fusion protein for pretargeted lymphoma therapy
Schultz, J., et al. (2000). Cancer Res. 2000 Dec 1;60(23):6663-9. PMID: 11118050
The B9E9 scFvSA fusion protein, derived from the CD20-specific murine IgG2a monoclonal antibody B9E9, was genetically engineered for pretargeted radioimmunotherapy. The V H and V L DNA fragments were amplified from B9E9 hybridoma cells, and the resulting scFvSA fusion protein showed nanomolar affinity to CD20-positive Ramos cells, comparable to the original B9E9 monoclonal antibody, as confirmed by in vitro and in vivo characterization.
Tags: B9E9 mAb; B9E9 monoclonal antibody
For more references about anti-human CD20 antibody (B9E9), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa
Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Human IgG1 Fc Silent Kappa
Syd Labs provides the following in vivo grade recombinant anti-human CD20 monoclonal antibodies:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)
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