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Recombinant Mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human CD20 Mouse IgG2b Kappa monoclonal antibody was produced in mammalian cells.
Clone: 2H7.
Isotype: Mouse IgG2b Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 2H7) specifically binds to human CD20.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD20 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human CD20 antibodies (clone of 2H7) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Two Are Better than One: The Bi-Specific Antibody Mosunetuzumab Enhances the Activity of Rituximab and Obinutuzumab in Preclinical Models
Budde, L. E., et al. (2024). Clin Cancer Res. 30(16):3502-3513. doi: 10.1158/1078-0432.CCR-24-0363. PMID: 39082712
The study evaluated the combination of mosunetuzumab, a CD20xCD3 T-cell engaging bispecific antibody, with rituximab or obinutuzumab in disseminated and subcutaneous NHL xenograft models. Mosunetuzumab redirects T cells to eliminate CD20-expressing B cells, and its combination with rituximab or obinutuzumab, both anti-CD20 monoclonal antibodies, resulted in greater tumor growth inhibition in Raji and WSU-DLCL2 xenografts compared to single-agent treatments. In vitro assessments further confirmed the synergistic effects observed in vivo.
Tags: anti-human CD20; activity of anti-human CD20 antibody
B cell depletion reduces T cell activation in pancreatic islets in a mouse model of type 1 diabetes
Boldison, J., et al. (2018). Diabetologia. 61(6):1393-1404. doi: 10.1007/s00125-018-4597-z. PMID: 29431641
In this study, mice were injected intraperitoneally with 250 μg of anti-CD20 mAb (clone 2H7) or control IgG2b (clone MPC-11) in 200 μl sterile PBS. Treatment with 2H7 efficiently depleted B cells from the spleen and pancreatic lymph nodes, as confirmed by flow cytometry, and significantly reduced diabetes incidence in NOD mice compared to controls. T cell activation markers (CD69 and CD44) were significantly reduced in the islets of 2H7-treated mice, suggesting that B cell depletion modulates T cell-mediated autoimmunity in pancreatic islets.
Tags: anti-human CD20 2H7 mAb; anti-human CD20 2H7 mAb in vivo
Tumor-Targeting Anti-CD20 Antibodies Mediate In Vitro Expansion and In Vivo Control of CD20+ T Cells in Hemophagocytic Lymphohistiocytosis
Locatelli, F., et al. (2018). Front Immunol. 9:1049. doi: 10.3389/fimmu.2018.01049. PMID: 29758324
The study tested anti-CD20 antibodies (2H7) in a murine model of hemophagocytic lymphohistiocytosis (HLH) using NSG mice reconstituted with human hematopoietic stem cells. Treatment with 2H7 significantly reduced CD20+ T cell populations in the spleen and bone marrow. In vitro, 2H7 induced apoptosis in CD20+ T cells, confirmed by Annexin V staining, highlighting the therapeutic potential of anti-CD20 antibodies in managing HLH by targeting aberrant T cell populations.
Tags: function of anti-human CD20 2H7; anti-human CD20 2H7 in cancer research
Development of Novel Anti-CD20 Monoclonal Antibodies and Engineering Strategies to Enhance Their Therapeutic Efficacy
Li, B., et al. (2016). Crit Rev Oncol Hematol. 105:37-47. doi: 10.1016/j.critrevonc.2016.03.001. PMID: 26957418
The study used the murine anti-CD20 mAb 2H7 as a reference to compare the binding affinity and therapeutic efficacy of newly engineered anti-CD20 antibodies. These new antibodies showed superior tumor growth inhibition, enhanced ADCC, and CDC activities compared to 2H7 in SCID mice engrafted with Raji cells. Flow cytometry confirmed the depletion of CD20+ cells in the peripheral blood and spleen of treated mice following intravenous administration of anti-CD20 antibodies.
Tags: bioactivity of anti-human CD20 2H7; anti-human CD20 (2H7) in vivo antibody
Combination Treatment With Anti-CD20 and Oral Anti-CD3 Prevents and Reverses Autoimmune Diabetes
Raz, I., et al. (2013). Diabetes. 62(8):2841-2849. doi: 10.2337/db12-1176. PMID: 23656886
Female NOD mice were treated with 250 μg of anti-CD20 antibody (2H7) via intraperitoneal injection, resulting in transient B cell depletion in the spleen and pancreatic lymph nodes, as confirmed by flow cytometry. Combination therapy with anti-CD20 and oral anti-CD3 significantly reduced diabetes incidence compared to single-agent treatments, increased regulatory T cell frequency in pancreatic islets, and was monitored through weekly blood glucose measurements.
Tags: anti-human CD20 antibody 2H7; clone 2H7 of anti-human CD20
For more references about anti-human CD20 antibody (2H7), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2b isotype control antibody
Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies:
In vivo grade recombinant anti-human CD20 antibodies (clone 2H7)
In vivo grade recombinant anti-human CD20 antibodies (clone B9E9)
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