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Recombinant Mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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The in vivo grade recombinant anti-human CD20 Mouse IgG2b Kappa monoclonal antibody was produced in mammalian cells.
Clone: 2H7.
Isotype: Mouse IgG2b Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 2H7) specifically binds to human CD20.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD20 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human CD20 antibodies (clone of 2H7) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Combination treatment with anti-CD20 and oral anti-CD3 prevents and reverses autoimmune diabetes.
Hu, C., et al. Diabetes. 2013 Aug;62(8):2849-58. doi: 10.2337/db12-1175. PMID: 23447122
Thus, the findings demonstrate that combining anti-CD20 and oral anti-CD3 is superior to anti-CD20 monotherapy for restoring normoglycemia in diabetic NOD mice, providing important preclinical evidence for the optimization of B cell–directed therapy for T1D. ...Thus, we tested the effect of intravenous anti-CD20 and oral anti-CD3 combined treatment for the prevention and reversal of T1D in the human CD20 transgenic NOD (hCD20/NOD) animal model. ...Herein, we show that oral administration of anti-CD3 together with intravenous injection of anti-CD20 has a synergistic effect on the prevention and reversal of T1D in the hCD20/NOD mouse. ...All fluorochrome-conjugated monoclonal antibodies (mAbs) were purchased from BioLegend, Inc. Affinity-purified mouse anti-hCD20 mAb 2H7 was prepared by Bio X Cell. ...To further improve the efficacy of treatment, we tested the combination treatment of anti-hCD20/oral anti-CD3 in hCD20/NOD mice.
Tags: anti-human CD20 2H7 mAb; anti-human CD20 2H7 mAb in vivo
B-cell depletion reactivates B lymphopoiesis in the BM and rejuvenates the B lineage in aging.
Keren, Z., et al. Blood. 2011 Mar 17;117(11):3104-12. doi: 10.1182/blood-2010-09-307983. PMID: 21228330
Mice used were C57Bl6 mice that are normal, or carrying a targeted conditional Baff-r locus (BAFF-RFL),25 transgenic for Mx-cre,26 R26R-EYFP transgene for the EYFP-Cre reporter system,27 or Balb/c transgenic for human CD20 (hCD20 Tg). ...An “old-like” B-cell phenotype in C57Bl6 mice and the hCD20Tg mice was determined when the frequency of newly generated AA4.1-expressing B cells in peripheral blood was less than 5% of the B220+ cells (supplemental Figure 4, available on the Blood Web site; see the Supplemental Materials link at the top of the online article). ...To deplete B cells in the hCD20 Tg model, mice were injected intraperitoneally with 1 mg/mouse of mouse anti–human CD20 monoclonal antibodies (clone 2H7) as described. ...To do so, we used transgenic mice expressing human CD20 specifically in the B lineage (hCD20 Tg mice), starting from the late pre-B stage. ...After B-cell depletion, complete B-cell reconstitution in hCD20 Tg mice lasted 60 to 70 days, as described.
Tags: anti-human CD20; activity of anti-human CD20 antibody
Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Polyak, M. J., et al. Blood. 2002 May 1;99(9):3256-62. doi: 10.1182/blood.v99.9.3256. PMID: 11964291
CD20, a nonglycosylated 33- to 35-kd integral membrane protein expressed at high density only on B lymphocytes, has proven to be an effective target for immunotherapeutic removal of malignant B cells. ...It is generally assumed, based on antibody blocking studies,5 that CD20 bears only 2 extracellular epitopes, one that is recognized by the vast majority of CD20 mAbs, and a second that is recognized by a single antibody (1F5) with unusual activating properties. ...Other CD20 mAbs were obtained from the human leukocyte differentiation workshops IV, VI, and VII. ...CD20 translocation experiments were performed as described.14 Briefly, cells were treated before lysis with anti-CD20 mAbs for 15 minutes at 37°C and both the cleared lysates and the insoluble pellets were collected for analysis by CD20 immunoblotting. ...Equal aliquots of each fraction were mixed with SDS sample buffer. Western blot analysis and Coomassie staining were used to identify the fractions containing CD20 and the molecular weight standards, respectively.
Tags: function of anti-human CD20 2H7; anti-human CD20 2H7 in cancer research
For more references about Anti-Human CD20 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2b isotype control antibody
Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies:
In vivo grade recombinant anti-human CD20 antibodies (clone 2H7)
In vivo grade recombinant anti-human CD20 antibodies (clone B9E9)
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