Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human CD20 Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
Clone: B9E9.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: B9E9) specifically binds to human CD20.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD20 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human CD20 antibodies (clone of B9E9) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Additional expression of T-cell engager in clinically tested oncolytic adeno-immunotherapy redirects tumor-infiltrated, irrelevant T cells against cancer cells to enhance antitumor immunity
Morita, D., et al. (2024). J Immunother Cancer. 2024 Dec 9;12(12):e009741. doi: 10.1136/jitc-2024-009741. PMID: 39653552
Fluorochrome-conjugated monoclonal antibodies, including anti-human CD20 (clone B9E9), were used to stain human cells for flow cytometry analysis with Gallios or BD FACSymphony cytometers at Texas Children’s Hospital. Eight weeks post-injection, PBMCs from humanized mice were stained with anti-human CD45 to confirm humanization. Other antibodies, such as anti-CD3 (UCHT1), CD4 (SK3), and CD8 (RPA-T8), were used to assess immune cell profiles, demonstrating T-cell redirection for enhanced antitumor immunity.
Tags: anti-human CD20 B9E9; anti-human CD20 B9E9 antibody
Phase 1 trial of a novel anti-CD20 fusion protein in pretargeted radioimmunotherapy for B-cell non-Hodgkin lymphoma
Forero, A., et al. (2004). Blood. 2004 Jul 1;104(1):227-36. doi: 10.1182/blood-2003-09-3284. PMID: 14996706
A phase 1 trial assessed the safety, pharmacokinetics, and immunogenicity of a tetrameric single-chain anti-CD20-streptavidin fusion protein (B9E9FP) in patients with B-cell non-Hodgkin lymphoma (NHL) for pretargeted radioimmunotherapy (PRIT). The B9E9FP (anti-human CD20) targeted HLA class I/peptide complexes, showing in vivo activity when combined with anti-viral T cells, supporting further clinical development with autologous CTLs from vaccination or ex vivo expansion.
Tags: anti-human CD20 B9E9 antibody in vivo; anti-human CD20 B9E9 in animal model
An oncolytic measles virus engineered to enter cells through the CD20 antigen
Bucheit, A. D., et al. (2003). Mol Ther. 2003 Jan;7(1):62-72. doi: 10.1016/s1525-0016(02)00033-3. PMID: 12573619
An oncolytic measles virus (MV) was engineered to enter CD20(+) target cells via a single-chain (scFv) anti-CD20 antibody (B9E9) interaction with the CD20 antigen in non-Hodgkin’s lymphoma (NHL) models. The virus demonstrated enhanced oncolytic activity in vivo against CD20(+) tumors, showing equivalent effects on CD20(-) tumors, highlighting the clinical relevance of B9E9 (anti-human CD20) for targeted viral therapy.
Tags: anti-human CD20 B9E9 antibody in animal model; anti-human CD20 B9E9 in cancer research
Anti-viral cytotoxic T cells inhibit the growth of cancer cells with antibody targeted HLA class I/peptide complexes in SCID mice
Savage, P., et al. (2002). Int J Cancer. 2002 Apr 1;98(4):538-44. doi: 10.1002/ijc.10219. PMID: 11920616
The B9E9 scFvSA fusion protein (anti-human CD20) targeted HLA-A2/M1 complexes to Daudi cells, enabling CTL-mediated killing in vitro at dilutions as low as 100 pg/ml. In vivo SCID mouse assays showed that only one of four mice receiving B9E9 scFvSA, HLA-A2/M1 complexes, and anti-HLA-A2/M1 CTLs developed a tumor, supporting clinical development with autologous CTLs.
Tags: anti-human CD20 B9E9; function of anti-human CD20 B9E9
Induction of viral and tumour specific CTL responses using antibody targeted HLA class I peptide complexes
Savage, P., et al. (2002). Br J Cancer. 2002 Apr 22;86(8):1336-42. doi: 10.1038/sj.bjc.6600223. PMID: 11953895
The B9E9 scFvSA fusion protein (anti-human CD20 mAb) delivered HLA-A2/class I peptide complexes to HLA class I-negative Daudi cells. Flow cytometry confirmed retention of targeted HLA-A2/M1 complexes, and in vitro CTL responses were observed in PBMCs pre-treated with B9E9 scFvSA. The B9E9 fusion protein, currently in clinical trials for B-cell lymphoma with radiolabeled biotin, demonstrated effective targeting for CTL induction.
Tags: bioactivity of anti-human CD20 B9E9; anti-human CD20 B9E9 antibody of low endotoxin
For more references about anti-human CD20 antibody (B9E9), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies:
In vivo grade recombinant anti-human CD20 antibodies (clone 2H7)
In vivo grade recombinant anti-human CD20 antibodies (clone B9E9)
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf