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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD20 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD20 Monoclonal Antibodies for Flow Cytometry

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, CD20

Catalog No. Product Name Size List Price (US$) Quantity
PA007465.m2b Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa 50 ug 40.00
PA007465.m2b Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa 200 ug 80.00
PA007465.h1Fs Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa 50 ug 80.00
PA007465.h1Fs Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa 200 ug 160.00
PA007746.h1Fs-b Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa 50 ug 65.00
PA007746.h1Fs-b Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa 200 ug 130.00
Description

PA007465.m2b: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa

Recombinant  Mouse IgG2b Monoclonal Antibody. 
Clone: 2H7.
Isotype:  Mouse IgG2b Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2H7 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007465.h1Fs: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 2H7.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2H7 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007746.h1Fs-b: Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 monoclonal antibody with site-directed  biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 2H7.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 2H7 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 2H7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human CD20 Monoclonal Antibody (Clone: 2H7)

H?rner, S., et al. Cancers (Basel). 2022 Aug 12;14(16):3941. doi: 10.3390/cancers14163941. PMID: 36010934
The study utilized hybridoma-derived antibodies 2H7, 4G7, and Apo-1 for quantification. The bispecific antibodies (bsAbs) combined the CD95 agonist Apo-1 with either the anti-CD20 antibody clone 2H7 or the anti-CD19 clone 4G7 in F(ab)2 and single-chain formats. The CD95xCD20 bsAb demonstrated remarkable potency with IC50 values as low as 25 pM, despite the relatively low affinity of the anti-CD20 mAb clone 2H7 as a single chain. Expression of CD20, CD19, and CD95 on various cell lines was quantified using flow cytometry with QIFIKIT calibration beads, and binding of bsAbs to Daudi and Jurkat cells was assessed via flow cytometry.
(Tags: 2H7 antibody; 2H7 antibody for cancer research)
Boldison, J., et al. Diabetologia. 2019 Sep;62(9):1669-1678. doi: 10.1007/s00125-019-04974-y. PMID: 31037349
In this study, female VH125.hCD20/NOD mice (6?8 weeks old) were treated with either anti-hCD20 antibody (clone 2H7) or control IgG2b antibody (clone MPC-11). Over a 12-week observation period, 48% of control-treated mice developed diabetes compared to 15% of 2H7-treated mice. The study involved injecting groups of 6- to 8-week-old VH125.hCD20/NOD mice with 2H7 anti-CD20 or IgG isotype control, followed by analysis of spleen and pancreatic lymph node (PLN) tissue for anti-insulin B cells post-treatment.
(Tags: 2H7 antibody for flow cytometry; 2H7 antibody mouse dose range)
For more technical references or data sheets regarding the Anti-Human CD20 Monoclonal Antibody (Clone: 2H7) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD20 monoclonal antibodies:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)
Recombinant Anti-human CD20 monoclonal antibody (Clone: 1F5)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD20 monoclonal antibodies:
Recombinant Anti-mouse CD20 monoclonal antibody (Clone: 2H7)

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7) for flow cytometry
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9) for flow cytometry

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