Anti-Human CD20 Monoclonal Antibodies for Flow Cytometry

PA007465.m2b: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa

Recombinant  Mouse IgG2b Monoclonal Antibody. 
Clone: 2H7.
Isotype:  Mouse IgG2b Kappa.
Source: The anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2H7 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007465.h1Fs: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 2H7.
Isotype: Human IgG1 kappa.
Source: The anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2H7 antibody specifically binds to human CD20.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 2H7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-human CD20 antibody (2H7):

IgG-Based Bispecific Anti-CD95 Antibodies for the Treatment of B Cell-Derived Malignancies and Autoimmune Diseases.
Hörner, S., et al. (2022). Cancers (Basel). 14(16):3941. doi: 10.3390/cancers14163941. PMID: 36010934
The study utilized hybridoma-derived antibodies 2H7, 4G7, and Apo-1 for quantification. The bispecific antibodies (bsAbs) combined the CD95 agonist Apo-1 with either the anti-CD20 antibody clone 2H7 or the anti-CD19 clone 4G7 in F(ab)2 and single-chain formats. The CD95xCD20 bsAb demonstrated remarkable potency with IC50 values as low as 25 pM, despite the relatively low affinity of the anti-CD20 mAb clone 2H7 as a single chain. Expression of CD20, CD19, and CD95 on various cell lines was quantified using flow cytometry with QIFIKIT calibration beads, and binding of bsAbs to Daudi and Jurkat cells was assessed via flow cytometry.
Tags: 2H7 antibody; 2H7 antibody for cancer research

Phenotypically distinct anti-insulin B cells repopulate pancreatic islets after anti-CD20 treatment in NOD mice.
Boldison, J., et al. (2019). Diabetologia. 62(9):1669-1678. doi: 10.1007/s00125-019-04974-y. PMID: 31037349
In this study, female VH125.hCD20/NOD mice (6–8 weeks old) were treated with either anti-hCD20 antibody (clone 2H7) or control IgG2b antibody (clone MPC-11). Over a 12-week observation period, 48% of control-treated mice developed diabetes compared to 15% of 2H7-treated mice. The study involved injecting groups of 6- to 8-week-old VH125.hCD20/NOD mice with 2H7 anti-CD20 or IgG isotype control, followed by analysis of spleen and pancreatic lymph node (PLN) tissue for anti-insulin B cells post-treatment.
Tags: 2H7 antibody for flow cytometry; 2H7 antibody mouse dose range

Targeted delivery of a PD-1-blocking scFv by CAR-T cells enhances anti-tumor efficacy in vivo.
Rafiq, S., et al. (2018). Nat Biotechnol. 36(9):847-856. doi: 10.1038/nbt.4195. PMID: 30102295
The study employed flow cytometry to quantify PD-1 expression on CAR-T cells, with representative dot plots showing decreased PD-1 detection on 1928z-E27 and 4H1128z-E27 T cells compared to second-generation CAR-T cells. Western blot analysis on flow-sorted CAR+ and CAR− T cell populations was conducted using anti-HA mAb. A unique peptide (FGSNLESGIPAR, m/z = 624.3226) from the EH12/2H7 antibody was identified via Skyline analysis, confirming its absence in mouse or human proteomes, supporting its use in characterizing the 2H7 anti-human CD20 antibody.
Tags: 2H7 mAb for flow cytometry; anti-human CD20 antibody (clone 2H7)

Expression of lectin-like transcript-1 in human tissues.
Llibre, A., et al. (2017). Oncotarget. 8(14):22800-22812. doi: 10.18632/oncotarget.15088. PMID: 28413611
The study evaluated a novel anti-LLT1 antibody (clone 2H7) for its staining capabilities in immunohistochemistry and flow cytometry, demonstrating effective recognition of lectin-like transcript-1 (LLT1) on germinal centre B cells. The 2H7 mAb, also an anti-human CD20 antibody, was used at a 1/500 dilution to stain human tissues (testes, brain, and placenta), proving its utility in studying LLT1 distribution through immunohistochemical staining.
Tags: anti-CD20 (2H7) monoclonal antibody; function of anti-human CD20 2H7

Development and Characterization of a New Antipeptide Monoclonal Antibody Directed to Human CD20 Antigen.
Habibi-Anbouhi, M., et al. (2015). Cancer Biother Radiopharm. 30(8):341-349. doi: 10.1089/cbr.2015.1863. PMID: 26352927
This study developed a new monoclonal antibody, 3B4C10, targeting chimeric human CD20 extra loops (hCD20 exl), which showed strong reactivity with both peptide and native antigens on Raji B-cell lines. The antibody’s balanced K(on) and K(off) suggest potential applications in affinity columns or beads for isolating CD20-positive cells and cancer stem cells. Another antibody, C12H mAb, demonstrated high affinity and reactivity with native antigens on Raji and Daudi cells in flow cytometry, highlighting its potential for immunotherapy targeting the B-cell CD20 antigen.
Tags: anti-human CD20 2H7 mAb for flow cytometry; anti-human CD20 2H7 antibody

For more references about anti-human CD20 antibody (2H7), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa
Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD20 monoclonal antibodies:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)