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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: FMC63.
Isotype: Mouse IgG2a kappa.
Source: The anti-human CD19 monoclonal antibody (clone: FMC63) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: FMC63) specifically binds to the human CD19 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The FMC63 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.
Solving the mystery of the FMC63-CD19 affinity
Seigner, J., et al. (2023). Sci Rep. 2023 Dec 27;13(1):23024. doi: 10.1038/s41598-023-48528-0. PMID: 38155191
The FMC63 antibody (anti-human CD19) is the basis for four of six FDA-approved CAR T cell therapies targeting CD19 for B-cell malignancies. Despite its widespread use, reported affinities of FMC63-scFv to CD19 vary from 0.3 to 47 nM. This study conducted a detailed affinity analysis of the FMC63-CD19 interaction to resolve these inconsistencies, highlighting its critical role in CAR T cell therapy development.
Tags: anti-human CD19 FMC63; anti-human CD19 FMC63 mAb
Safety and efficacy of a novel anti-CD19 chimeric antigen receptor T cell product targeting a membrane-proximal domain of CD19 with fast on- and off-rates against non-Hodgkin lymphoma: a first-in-human study
Zhang, Y., et al. (2023). Mol Cancer. 2023 Dec 9;22(1):200. doi: 10.1186/s12943-023-01886-9. PMID: 38066564
The FMC63 scFv (anti-human CD19) is used in all four commercial CAR T cell products, targeting a membrane-distal CD19 epitope with slow on- and off-rates. A novel anti-CD19 scFv (h1218) targeting a membrane-proximal epitope with faster kinetics outperformed FMC63-CAR T cells in eradicating lymphomas with CD19 mutations (L174V, R163L) in vivo, showing reduced activation-induced cell death and enhanced expansion.
Tags: anti-human CD19 FMC63 antibody in vivo; anti-human CD19 FMC63 in animal model
Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus
Rive, C. M., et al. (2022). Mol Ther Methods Clin Dev. 2022 Sep 9;26:205-218. doi: 10.1016/j.omtm.2022.05.006. PMID: 35755944
A second-generation anti-CD19 CAR with FMC63 scFv (anti-human CD19) showed robust B-cell targeting in vitro, with 18.5% ± 1.08% of murine B cells unviable after 5-hour co-culture. Compared to 1D3 (anti-mouse CD19), FMC63-CAR T cells achieved an 8.8-fold expansion in transduced splenocytes, demonstrating selective and effective B-cell depletion in vivo using a standard CAR design.
Tags: anti-human CD19 FMC63 mAb in animal model; anti-human CD19 FMC63 in cancer research
Fully human CD19-specific chimeric antigen receptors for T-cell therapy
Sommermeyer, D., et al. (2017). Leukemia. 2017 Oct;31(10):2191-2199. doi: 10.1038/leu.2017.57. PMID: 28202953
Human CD19-specific scFvs with binding characteristics similar to FMC63 (anti-human CD19) were generated from human Ab/DNA-libraries, targeting an overlapping CD19 epitope. These scFvs were incorporated into CARs and compared to FMC63-based CARs, which are used in clinical trials. Flow cytometry with FMC63 competition confirmed their binding specificity, demonstrating comparable T-cell redirection for B-cell malignancy treatment.
Tags: anti-human CD19 FMC63 mAb in cancer research; anti-human CD19 (FMC63) in vivo antibody
For more references about anti-human CD19 antibody (FMC63), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)
Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)
Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry
Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry
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