Anti-biotin Antibodies

Background of Recombinant Anti-biotin Antibody

Recombinant mouse anti-biotin antibodies recognize biotin (vitamin B7) attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.

The anti-biotin monoclonal antibody can be used as a second-step reagent in various applications for detecting biotinylated targets. The biotin-streptavidin system is widely used in immunoassay signal amplification techniques because streptavidin binds biotin with extremely high affinity.

Because biotin is naturally present in a large variety of biological samples, it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. Streptavidin/biotin blocking kit, avidin/biotin blocking system, or biotin blockers are used to block signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.

Please contact us to ask for a quote for the engineered anti-biotin antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-biotin antibodies and mutants are available.

PA007522.h1: In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa

The in vivo grade recombinant anti-biotin antibody, human IgG1 kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Human IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

PA007522.h1IHH: In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1-I253A H310A H435A (IAHAHA) Kappa

The in vivo grade recombinant anti-biotin antibody, human IgG1-IAHAHA kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Human IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

PA007522.m1: In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG1 Kappa

The in vivo grade recombinant anti-biotin antibody, mouse IgG1 kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Mouse IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

Shipping: The in vivo grade recombinant anti-biotin antibodies (clone of F1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20 to -70°C as supplied.

References of Anti-biotin Antibodies:

I?B? controls dormancy in hematopoietic stem cells via retinoic acid during embryonic development.
Thambyrajah, R., et al. Nat Commun. 2024 Jun 1;15(1):4673. doi: 10.1038/s41467-024-48854-5. PMID: 38824124.
Cells were washed with PBS?+?5%FBS and then labeled with a cocktail of antibodies against lineage markers before conjugation to anti-Biotin microBeads. ...The fetal liver was harvested from E14.5 embryos and depleted from lineage-primed progenitors as described above. ...Altogether, we postulate that LT-HSCs must interpret the inflammatory signals that they receive in the AGM differently to HPC through the nuclear I?B?-PRC2 complex. ...In support of the molecular profiling, I?B? KO LT-HSCs cycle less as quantified by Ki67 staining and BrdU labeling experiments. ...Recent findings suggest that Hematopoietic Stem Cells (HSC) and progenitors arise simultaneously and independently of each other already in the embryonic aorta-gonad mesonephros region, but it is still unknown how their different features are established.
Tags: activity of Anti-biotin; activity of Anti-biotin mAb

B cell-based therapy produces antibodies that inhibit glioblastoma growth.
Wang, S., et al. J Clin Invest. 2024 Aug 29;134(20):e177384. doi: 10.1172/JCI177384. PMID: 39207859.
TIB cells were magnetically isolated using CD19-biotin and anti-biotin Microbeads. ...Most TIB target antigens identified in patient tissue samples span across the nuclear, cytoplasmic, and extracellular compartments. ...Glioblastoma (GBM) is a highly aggressive and malignant brain tumor with limited therapeutic options and a poor prognosis. ...In this study, we aimed to characterize the antigenic reactivity of BVax-derived Abs and evaluate their therapeutic potential. ...Proteomics analyses indicated that the Abs produced by BVax had unique reactivity, predominantly targeting factors associated with cell motility and the extracellular matrix.
Tags: Anti-biotin antibody for cancer research; Anti-biotin for animal model

The effector program of human CD8 T cells supports tissue remodeling.
Delacher, M., et al. J Exp Med. 2024 Feb 5;221(2):e20230488. doi: 10.1084/jem.20230488. PMID: 38226976.
Cells were pre-enriched with biotinylated anti-human CD8, followed by column-based magnetic separation with anti-biotin ultrapure microbeads following the manufacturer’s protocol. ...Whether human CD8 T cells can, in parallel, induce a tissue regeneration program is poorly understood. ...Activated CD8 T cells could produce the epidermal growth factor receptor (EGFR)-ligand amphiregulin (AREG) and sensitize epithelial cells for enhanced regeneration potential. ...Using single-cell gene expression analysis, we identified an AREG+, tissue-resident CD8 T cell population in skin and adipose tissue from patients undergoing abdominal wall or abdominoplasty surgery. ...Tissue regeneration and remodeling are critical functions to respond to tissue damage caused by infection, inflammation, trauma, or other insults, but excessive repair can also lead to fibrosis and support tumor growth.
Tags: Anti-biotin for mouse tumor model; Anti-biotin dose

Anti-CD19 CAR-T cells are effective in severe idiopathic Lambert-Eaton myasthenic syndrome.
Wickel, J., et al. Cell Rep Med. 2024 Nov;5(11):101794. doi: 10.1016/j.xcrm.2024.101794. PMID: 39447569.
After removal of the supernatant, cells were stained with 50?L of APC-coupled anti-Biotin antibody for 15min on ice. ...As we report a single case without a control patient, no statistical analyses were performed. ...Baseline and follow-up clinical assessments were performed by the same neurologist experienced in the treatment of myasthenic syndromes. ...Care was taken to carry out the assessments at the same time of day. MG-ADL was completed by the patient. ...Data presented here are based on a single case report without an adequate control due to the treatment in a compassionate use setting.
Tags: Anti-biotin antibody mouse dose range; Anti-biotin in vivo antibody

Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females.
Kumar, A., et al. Redox Biol. 2024 Feb;69:102986. doi: 10.1016/j.redox.2023.102986. PMID: 38091879.
The cells were incubated with anti-biotin and Tom-20 antibodies diluted in 5 % bovine serum albumin (BSA) and left overnight at 4°C in the dark. ...The cells were washed at least three times with 1 % BSA after each primary and secondary incubation step. ...Images were acquired using Leica SP8 confocal microscope (Leica microsystems), the cells were analyzed for Pearson's correlation, and the R-value was determined using Coloc 2 plugin in ImageJ software. ...The tissues were washed and fixed with 4 % PFA overnight at 4 °C followed by the same staining process and concentrations described above. ...Mice were anesthetized as described earlier, and the corneal images were taken using a slit lamp biomicroscope attached to a camera.
Tags: Anti-biotin recombinant isotype control; Anti-biotin for cancer research

For more references about Anti-biotin Antibodies please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-Biotin antibodies:
In Vivo Grade Recombinant Anti-Biotin Antibodies (Clone F1)

Syd Labs provides the following recombinant anti-Fluorescein/Digoxin/Biotin/Phospho-Tyrosine monoclonal antibodies:
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4-4-20 (enhanced))
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4M5.3)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 5A7)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 26-10)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone M33)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone Py20)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone 4G10)