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Anti-biotin Antibodies

Biotin; Vitamin H; Vitamin B7

Catalog No. Product Name Size List Price (US$) Quantity
PA007522.h1 In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa 1 mg 300.00
PA007522.h1 In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa 5 mg 650.00
PA007522.h1 In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa 25 mg 1500.00
PA007522.h1IHH In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1-I253A H310A H435A (IAHAHA) Kappa 1 mg 300.00
PA007522.h1IHH In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1-I253A H310A H435A (IAHAHA) Kappa 5 mg 650.00
PA007522.h1IHH In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1-I253A H310A H435A (IAHAHA) Kappa 25 mg 1500.00
PA007522.m1 In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG1 Kappa 1 mg 300.00
PA007522.m1 In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG1 Kappa 5 mg 650.00
PA007522.m1 In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG1 Kappa 25 mg 1500.00
PA007522.m2a In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa 1 mg 300.00
PA007522.m2a In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa 5 mg 650.00
PA007522.m2a In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa 25 mg 1500.00
Description

Background of Recombinant Anti-biotin Antibody

Recombinant mouse anti-biotin antibodies recognize biotin (vitamin B7) attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.

The anti-biotin monoclonal antibody can be used as a second-step reagent in various applications for detecting biotinylated targets. The biotin-streptavidin system is widely used in immunoassay signal amplification techniques because streptavidin binds biotin with extremely high affinity.

Because biotin is naturally present in a large variety of biological samples, it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. Streptavidin/biotin blocking kit, avidin/biotin blocking system, or biotin blockers are used to block signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.

Please contact us to ask for a quote for the engineered anti-biotin antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-biotin antibodies and mutants are available.

PA007522.h1: In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa

The in vivo grade recombinant anti-biotin antibody, human IgG1 kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Human IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

PA007522.h1IHH: In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1-I253A H310A H435A (IAHAHA) Kappa

The in vivo grade recombinant anti-biotin antibody, human IgG1-IAHAHA kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Human IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

PA007522.m1: In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG1 Kappa

The in vivo grade recombinant anti-biotin antibody, mouse IgG1 kappa was produced in mammalian cells.
Specificity: Biotin.
Clone: F1.
Isotype: Mouse IgG1 kappa.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form of Antibody: 0.2 uM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.

Shipping: The in vivo grade recombinant anti-biotin antibodies (clone of F1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20 to -70°C as supplied.

Syd Labs provides the following in vivo grade recombinant anti-Biotin/DNP/Fluorescein antibodies:
In Vivo Grade Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
In Vivo Grade Recombinant Anti-DNP Monoclonal Antibodies (Clone DNP.m1)
In Vivo Grade Recombinant Anti-Fluorescein (FITC) Monoclonal Antibodies (Clone 4-4-20 (enhanced))

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