BP004993: Biotinylated Human Nectin-1/PVRL1/CD111 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein, (amino acids Gln31-Thr334) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Nectin-3 protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human Nectin-1 protein with the C-His tag with the EC50 of 0.52 ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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