BP004993: Biotinylated Human Nectin-1/PVRL1/CD111 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein, (amino acids Gln31-Thr334) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Nectin-3 protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human Nectin-1 protein with the C-His tag with the EC50 of 0.52 ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000559-ENZ-479: Recombinant Human ALDH3A1
Source: E. coli-derived.
Specific activity is < 1 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols.
Purity > 95%, by SDS-PAGE.