BP004993: Biotinylated Human Nectin-1/PVRL1/CD111 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein, (amino acids Gln31-Thr334) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Nectin-3 protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human Nectin-1 protein with the C-His tag with the EC50 of 0.52 ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004686: Human MARCO Protein with the N-His Tag
Source: The recombinant Human MARCO Protein, (amino acids Ser405-Val520) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000558-ENZ-401: Recombinant Human Aldehyde Dehydrogenase (ALDH2)
Source: E. coli-derived.
Specific activity was found to be > 0.14 units/ml. Activity was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. 1 unit will oxidize 1umole of acetaldehyde to acetic acid per minute at pH 8 at 25C in the presence of beta-NAD, potassium and thiols.
Purity > 90%, by SDS-PAGE.