BP003436: Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide) fused with a His-Avi tag at the C-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer protein with the C-His tag with the EC50 of 7.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005188: Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer protein with the C-His tag with the EC50 of 8.4 ng/ml determined by ELISA (QC Test). The affinity constant of 13.43 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.