BP004987: Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated human PD-1 with the His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005587: Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated recombinant human PD-1/PDCD1 protein with the hFc-Avi tag to the immobilized human PD-L1 with the mFc tag at 2 ug/ml (100 ul/Well) was 0.19 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005136: Mouse PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant mouse PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via anti-His antibody can bind to the recombinant mouse PD-1 with the C-hFc Tag with an affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.PA000123-R12-2432: ABL1 Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, IHC, ELISA;
Reactivity: Human, Mouse, Rat.