Name: VHH Isotype Control Antibodies
Brand: Syd Labs
SKU: PA007165.His
Price: 350.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007165.His

In Vivo Grade Recombinant Anti-HEL VHH-His Isotype Control Antibody

1 mg

350.00

PA007165.h1

In Vivo Grade Recombinant Anti-HEL VHH-hIgG1 Fc Isotype Control Antibody

1 mg

350.00

PA007165.m1

In Vivo Grade Recombinant Anti-HEL VHH-mIgG1 Fc Isotype Control Antibody

1 mg

350.00

PA007165.m2a

In Vivo Grade Recombinant Anti-HEL VHH-mIgG2a Fc Isotype Control Antibody

1 mg

350.00

PA007165.hh1

In Vivo Grade Recombinant Humanized Anti-HEL VHH-hIgG1 Fc Isotype Control Antibody

1 mg

350.00

Description

Background

The anti-HEL VHH (hen egg-white lysozyme; nanobody, single-domain antibody, or the variable domain of heavy chain antibody) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. As an ideal recombinant VHH isotype control antibody, the recombinant anti-HEL VHH isotype control antibody has low or no specific binding to any human and mouse sample. The recombinant anti-HEL VHH isotype control antibodies are useful for in vitro and in vivo studies from ELISA, cell-based assay to animal model study.

Please contact us to ask for a quote for the engineered anti-HEL VHH isotype control antibodies with His- and Fc-tags. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.

PA007165.His: In Vivo Grade Recombinant Anti-HEL VHH-His Isotype Control Antibody

The in vivo grade recombinant anti-HEL VHH isotype control antibody with the His-tag was produced in mammalian cells.
Specificity: hen egg-white lysozyme (HEL).
Clone: D3-L11.
Isotype: VHH.
Applications: an isotype-matched negative control for anti-HEL VHH antibody used in ELISA, Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007165.h1: In Vivo Grade Recombinant Anti-HEL VHH-hIgG1 Fc Isotype Control Antibody

The in vivo grade recombinant anti-HEL VHH isotype control antibody with the hIgG1 Fc-tag was produced in mammalian cells.
Specificity hen egg-white lysozyme (HEL).
Clone: D3-L11.
Isotype: VHH.
Applications: an isotype-matched negative control for anti-HEL VHH antibody used in ELISA, Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-HEL VHH isotype control antibodies (clone of D3-L11) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Anti-HEL VHH-His Isotype Control Antibodies:

Development of novel humanized VHH synthetic libraries based on physicochemical analyses.
Kondo, D., et al. Sci Rep. 2024;14:19013. doi: 10.1038/s41598-024-70513-4. PMID: 39168600
This study focuses on heavy-chain antibodies (HCAbs), which lack the light chain and the first heavy chain constant region. The variable antigen-binding domain (VHH) of HCAbs, with a size of approximately 15 kDa, remains functional as a single domain. Novel synthetic humanized VHH libraries were designed and constructed using the complementarity determining region-grafting method and comprehensive sequence analyses of VHHs from the protein data bank. Due to their high affinity and specificity, VHHs are an attractive alternative to conventional fragment antibodies for various antibody-based applications.
Tags: activity of anti-HEL VHH-His isotype control antibody; anti-HEL VHH-His isotype control

The development of single-domain VHH nanobodies that target the Candida albicans cell surface.
Pfaller, M. A., et al. Microbiol Spectr. 2024;12(10):e0174724. doi: 10.1128/spectrum.01747-24. PMID: 39373478
This study measured the mean fluorescence intensity (MFI) for C. albicans als4? mutant and SC5314 strain, in various conditions, immunolabeled with VHH19, isotype control (#34), no VHH control, and detection antibody-only control. VHH libraries were panned against echinocandin-resistant C. albicans isolates and counter-selected against susceptible isolates to isolate binding domains specific for drug-resistant cells. Thirty diverse VHH nanobodies were selected, and their binding characteristics were assessed via dose-response ELISA, showing specificity for C. albicans across various isolates and other Candida species.
Tags: anti-HEL VHH-His isotype control mAb; anti-HEL VHH-His isotype control antibody for animal model

VHH CDR-H3 conformation is determined by VH germline usage.
Fridy, P. C., et al. Commun Biol. 2023;6:864. doi: 10.1038/s42003-023-05241-y. PMID: 37604950
This study assembled paired consensus R1 and R2 reads into a single sequence spanning the VHH and part of the hinge region using the flash pairwise aligner. IGHV, IGHD, and IGHJ germlines were assigned using IgBlast with the IMGT-published alpaca reference germline set. Constant domain isotypes were identified using llama hinge-derived sequences with 100% sequence pattern matching. VHHs, or nanobodies, are single antigen-binding domains from camelid heavy-chain antibodies, widely used as diagnostic, research, and therapeutic tools.
Tags: anti-HEL VHH-His isotype control antibody for mouse tumor model; anti-HEL VHH-His isotype control for animal model

Applications and challenges in designing VHH-based bispecific antibodies: leveraging machine learning solutions.
Wang, Y., et al. MAbs. 2023;15(1):2210444. doi: 10.1080/19420862.2023.2210444. PMID: 37140123
This study highlights the role of machine learning in enhancing lead discovery, optimization, and structural prediction for therapeutic VHHs, though its application lags behind that in conventional antibody development. The development of bispecific antibodies, which bind at least two different targets, requires integrating multiple binding domains with distinct properties. The study emphasizes the need for high-quality, domain-specific data to support fragment interaction analysis and explores machine learning approaches tailored for VHH-enabled drug discovery, particularly in oncology, autoimmune diseases, and inflammation.
Tags: anti-HEL VHH-His isotype control for cancer research; anti-HEL VHH-His isotype control for mouse tumor model

First-in-class trispecific VHH-Fc based antibody with potent prophylactic and therapeutic efficacy against SARS-CoV-2 and variants.
Dennison, S. M., et al. Sci Rep. 2022;12:3921. doi: 10.1038/s41598-022-07952-4. PMID: 35260609
This study evaluated the binding of ABS-VIR-001, a trispecific VHH-Fc antibody, to SARS-CoV-2 S RBD using high-binding 96-well plates coated with 1 ?g/ml of S RBD. ABS-VIR-001 and a VHH-Fc cocktail were serially diluted and tested in ELISA assay buffer. Binding and inhibition were assessed using a probe loaded with ACE-2-b and S protein (wild-type or mutants) in the presence or absence of ABS-VIR-001, with association curves generated by GatorPrime software to demonstrate qualitative binding and inhibition signals.
Tags: function of anti-HEL VHH-His isotype control antibody; anti-HEL VHH-His isotype control in vivo antibody

For more references about anti-HEL VHH-His isotype control antibodies please contact our scientific support team with message@sydlabs.com.

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PA007165.His: In Vivo Grade Recombinant Anti-HEL VHH-His Isotype Control Antibody

PA007165.h1: In Vivo Grade Recombinant Anti-HEL VHH-hIgG1 Fc Isotype Control Antibody

References of Anti-HEL VHH-His Isotype Control Antibodies: