- IA000043-P-9002-002
R_PE_antibody bioconjugation technology is superior to the maleimido/thiol_based method as it is:
More efficient: Greater than 95% of antibody is converted to conjugate.
More easily purified: In most cases, the percent conversion of free antibody to conjugate is 100%, leaving no unconjugated antibody. Therefore it is only necessary to purify out the excess R_PE to obtain a purified conjugate. In many applications even, purification is not necessary.
Controllable: The level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic_modification on the antibody. In flow cytometry applications, heterodimer product is required and preparation of this construct can be easily optimized using this technology.
Gentle on Antibodies: Other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. Our technology, however, gently incorporates HyNic moieties on the intact antibody.
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