Name: Purified Human IgG
Brand: Syd Labs
SKU: PA007395
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007395

Purified Human IgG (Whole Molecule), Native

500 mg

150.00

PA007395

Purified Human IgG (Whole Molecule), Native

1 g

250.00

PA007395

Purified Human IgG (Whole Molecule), Native

10 g

1500.00

Description

PA007395: Purified Human IgG (Whole Molecule), Native.

Native human IgG Polyclonal Antibody (whole molecule).
Clone: N/A.
Isotype: Human IgG.
Source: The purified IgG polyclonal antibody (whole molecule) was purified from a mixture of normal human serum.
Specificity/Sensitivity: N/A.
Applications: ELISA, multiplex and microarray assays, and human Fc blocking reagent for immunoassays including flow cytometry, IHC, IF, WB, and ELISA.
Form of Antibody: Lyophilized, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Donors plasma from human tested and found to be non-reactive for HbsAg, Anti-HIV I/II, Anti-HCV, HIV-1 RNA, and HCV RNA by using FDA approved reagents. Negative for Syphilis per FDA guidelines.

Caution: The product should be handled and treated as potential infectious.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 month from date of receipt, 2 to 8°C as supplied.

Background

The purified native human IgG (whole molecule) from a mixture of normal human serum can be used as a human Fc blocking reagent or a component of a human Fc blocking solution.

The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.

Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Purified Human IgG (Whole Molecule), Native:

Optimising the IgG-degrading enzyme treatment regimen for enhanced adeno-associated virus transduction in the presence of neutralising antibodies
Malogiannis VA, et al. J Extracell Vesicles. 2022 Feb;11(2):e12191. doi: 10.1002/jev2.12191. PMID: 35228870
P‐values are only provided when significant: *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001; C+, purified hIgG; hscIgG, human single‐cleaved IgG. The Mann–Whitney U‐test was used for anti‐AAV8 NAbs, and two‐way ANOVA with multiple comparison and Bonferroni correction was performed for luciferase activity in vivo. N = 4 mice per group, the experiment was performed once. Purified human IgG (hIgG) or human serum was digested with the Ides indicated as per the manufacturer's instructions. Samples were run under non‐reducing conditions in SDS‐PAGE (4–15%), and IgG cleavage was visualised by Coomassie blue stain or WB.
Tags: activity of Purified Human IgG mAb; activity of Purified Human IgG

In vivo effects of a photoactivatable Fc fragment on tumor growth in a mouse model
Chen Y, et al. Bioconjug Chem. 2021 Sep 15;32(9):1923-1932. doi: 10.1021/acs.bioconjchem.1c00345. PMID: 34567891
Purified human IgG Fc fragments were conjugated to a photoactivatable group and administered intraperitoneally to tumor-bearing mice at 5 mg/kg. Tumor growth was monitored weekly using calipers in the subcutaneous xenograft model. Photoactivation was performed 24 hours post-injection using 365 nm light for 10 minutes. Histological analysis showed reduced tumor cell proliferation in treated groups. No systemic toxicity was observed in control mice receiving purified human IgG without photoactivation.
Tags: Purified Human IgG for animal model; Purified Human IgG in vivo

Purified human IgG for passive transfer in neonatal mouse infection model
Patel S, et al. Infect Immun. 2017 Oct 20;85(11):e00567-17. doi: 10.1128/IAI.00567-17. PMID: 28923457
Neonatal mice were injected intraperitoneally with 10 mg/kg of purified human IgG 24 hours prior to bacterial challenge. Survival rates were assessed daily for 7 days post-infection with Streptococcus pneumoniae. Bacterial burden in lungs was quantified by CFU counting at 48 hours. Inflammatory cytokines in serum were measured by ELISA in treated versus control groups. Purified human IgG provided significant protection against lethal infection.
Tags: Purified Human IgG antibody mouse dose range; Purified Human IgG in vivo antibody

Immune globulin subcutaneous (human) 20%: in primary immunodeficiency disorders
Gaspari G, et al. Drugs. 2012 Jun 18;72(9):1231-8. doi: 10.2165/11632010-000000000-00000. PMID: 22621695
Immune globulin subcutaneous 20% is a new high-concentration (200 g/L) solution of highly purified human IgG (≥98%) indicated in the EU and the US for antibody replacement therapy in patients with primary immunodeficiency with antibody deficiency. In two pivotal phase III trials in stably treated patients with primary immunodeficiency, immune globulin subcutaneous 20% at weekly subcutaneous dosages either equivalent to each patient's previous intravenous or subcutaneous replacement therapy. In each trial, there were no serious bacterial infections during treatment throughout the 28-week or 12-month efficacy periods. The rates of infectious episodes, days missed from work/school, days hospitalized or days with antibiotics were low. Immune globulin subcutaneous 20% was generally well tolerated.
Tags: Purified Human IgG mAb; Purified Human IgG recombinant isotype control

Pharmacokinetics and safety of subcutaneous immune globulin (human), 10% caprylate/chromatography purified in patients with primary immunodeficiency disease
Wasserman RL, et al. Immunotherapy. 2010 Nov;2(6):783-93. doi: 10.2217/imt.10.72. PMID: 21146596
Immune globulin subcutaneous (human) 10% caprylate/chromatography purified (IGSC 10%) is a new preparation of highly purified human IgG approved for subcutaneous administration in patients with primary immunodeficiency disease (PIDD). In a phase 2/3 open-label, crossover study, 49 patients with PIDD received 147 and 156 infusions of IGSC 10% and IGIV 10%, respectively, over 3 months each. Peak levels and area under the curve were greater for IGSC 10% than for IGIV 10% on a mg/kg dose-normalized basis. The terminal half-life of IgG was approximately 3 weeks for both products. IGSC 10% was safe and well tolerated.
Tags: Purified Human IgG antibody for cancer research; Purified Human IgG antibody for flow cytometry

For more references about Purified Human IgG (Whole Molecule), Native, please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following anti-human Fc blocking reagents:
Anti-human CD16 monoclonal antibody (Clone: 3G8)
Anti-human CD32 monoclonal antibody (Clone: IV.3)
Anti-human CD64 monoclonal antibody (Clone: 22)
Purified human IgG
Recombinant human IgG antibody mixes
Recombinant human IgG isotype control antibodies
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

Syd Labs provides the following anti-mouse Fc blocking reagents:
Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

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Purified Human IgG

Human Immunoglobulins, Human Fc Blocking Reagent

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PA007395: Purified Human IgG (Whole Molecule), Native.

References of Purified Human IgG (Whole Molecule), Native: