100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).
Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by Sanger DNA sequencing or next generation sequencing (NGS) has been successfully applied to single B cell antibody technologies.
The antibody variable regions of both heavy chain (VH) and light chain (VL) are analyzed for liabilities including, but not limited to N-linked glycosylation, deamidation, isomerization, pyroglutamate formation, and oxidation.
Fast turnaround time: 1 week per antibody, but may require more time depending on the complexity of analysis.N-terminal Edman sequencing of proteins or peptides can be used to determine the N-terminal amino acid residues of purified protein samples in addition to cleavage sites, and to identify an unknown protein or confirm the identity of a known protein, or to design oligonucleotide probes.
High sensitivity: even with picomoles of protein.
Various formats: in solution, gels or on PVDF blots.
Fast turnaround time: 1-2 weeks per sample, but may require more time depending on the complexity of analysis.