BP000092-GPF-001: Recombinant Murine Leukemia Inhibitory Factor (LIF) Protein, GST tag
Source: E. coli-derived.
mLIF supplement is assessed both on mESCs and on murine M1 myeloid leukemic cells. A standard of 50 units is defined as the concentration of mLIF supplement in 1.0 ml of tissue culture medium that induces the differentiation of 50% of M1 colonies (Metcalf, 1988).
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/ml.
Murine myeloid leukemic M1 Assay: Specific Activity >108 units/mg.
Purity: > 95% by SDS-PAGE.
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