BP004282: Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer Protein, (amino acids Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M)and RMFPNAPYL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody protein with the C-hFc tag with the EC50 of 6.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005373: Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody protein with the C-hFc tag with the EC50 of 19.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.33 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005272: Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody protein with the C-hFc tag with the EC50 of 1.8 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005768: Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody protein with the C-hFc tag with the EC50 of 14.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.APIM050346: WT161 (WT-161)
CAS No.: 1206731-57-8.
Purity: >98% by HPLC at 254nm.
QC: HPLC-MS, NMR, and quantitative elemental analysis report.
Solubility: Refer to Certificate.BP004283: Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein, (amino acids Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and RMFPNAPYL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody protein with the C-hFc tag with the EC50 of 9.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.