Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.
A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.
Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.
BP004932: Biotinylated Human IL-1 Beta/IL-1F2 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein, (amino acids Ala117-Ser269) fused with a His-Avi tag at the C-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-1R2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta protein with the C-His tag with the EC50 of 13.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005753: Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein, (amino acids Asp20-Arg335) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-IL-1 Rrp-2 Antibody protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Rrp2 protein with the C-His tag with the EC50 of 46.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005646: Biotinylated Human IL-1R3/IL-1 RAcP Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein, (amino acids Ser21-Glu359) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human IL-1R3 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-IL-1R3 Antibody protein with the C-hFc tag with the EC50 of 2.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004199: Biotinylated Human SOST/Sclerostin Protein with the N-His-Avi Tag
Source: The recombinant Biotinylated Human SOST/Sclerostin Protein, (amino acids Gln24-Tyr213) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-SOST Antibody protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human SOST protein with the N-His tag with the EC50 of 9.6 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP002641-CG19: Recombinant Human Caspase-14 (CASP14) Enzyme
Source: E. coli-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP003933: Cynomolgus IL-1 alpha/IL-1A Protein with the C-His Tag
Source: The recombinant Cynomolgus IL-1 alpha/IL-1A Protein, (amino acids Ser113-Ala271) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003661: Cynomolgus IL-1 Beta/IL-1F2 Protein with the C-His Tag
Source: The recombinant Cynomolgus IL-1 Beta/IL-1F2 Protein, (amino acids Ala117-Ser268) fused with a His tag at the C-terminus, was produced in E.coli cells.
Activity: The affinity constant of 1.40 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004403: Cynomolgus IL-1 Rrp2/IL-1 R6 Protein with the C-His Tag
Source: The recombinant Cynomolgus IL-1 Rrp2/IL-1 R6 Protein, (amino acids Asp20-Arg335) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003381: Cynomolgus IL-1R3/IL-1 RAcP Protein with the C-His Tag
Source: The recombinant Cynomolgus IL-1R3/IL-1 RAcP Protein, (amino acids Ser21-Cys362) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus IL-1R3 protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IL-1R3 Antibody protein with the C-hFc tag with the EC50 of 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.