Anti-Mouse CD40 Monoclonal Antibodies

PA007274.r2a: In vivo Grade Recombinant Anti-mouse CD40 Monoclonal Antibody, Rat IgG2a Kappa (Clone: FGK4.5)

Recombinant rat IgG2a Monoclonal Antibody. 
Clone: FGK4.5 / FGK45.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: FGK4.5) specifically binds to the mouse CD40 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD40 protein. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007274.m2a: In vivo Grade Recombinant Anti-mouse CD40 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: FGK4.5)

Recombinant mouse IgG2a Monoclonal Antibody. 
Clone: FGK4.5 / FGK45.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: FGK4.5) specifically binds to the mouse CD40 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD40 protein. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The FGK4.5 antibody binds to the cell-surface molecule CD40, a member of the tumor necrosis factor receptor superfamily. CD40 is expressed by antigen-presenting cells (APC) and many tumor cells.

Related Recombinant IgG Reference Antibodies:
Recombinant rat lgG2a isotype control antibody
Recombinant mouse IgG2a isotype control antibody

References of anti-mouse CD40 antibody (Clone: FGK4.5):

FGK4.5-mediated CD40 activation in esophageal cancer mouse models
Ashe, S., et al. Front Immunol. 2022 Oct 14;13:1010383. doi: 10.3389/fimmu.2022.1010383. PMID: 36238292
Mice were injected intraperitoneally with 10 µg of the agonistic anti-mouse CD40 antibody FGK4.5 (BioXCell, West Hanover, NH, #BE0016) or rat IgG2a isotype control every Tuesday and Friday for 2.5 weeks. Tumor burden was assessed by histological analysis, showing reduced esophageal lesions in FGK4.5-treated mice. Flow cytometry analyses demonstrated increased CD8+ T cell infiltration in tumors post-FGK4.5 treatment. Survival analysis indicated a 35% increase in median survival in the FGK4.5 group compared to controls (P < 0.05). FGK4.5 treatment upregulated IL-12 production in tumor-associated dendritic cells, as measured by ELISA.
Tags: anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb

Anti-mouse CD40 (FGK4.5) in chronic viral infection models
Lee, K., et al. J Immunol. 2023 Dec 1;211(12):2345-2356. doi: 10.4049/jimmunol.2300456. PMID: 38133142
FGK4.5 antibody (200 μg i.p., BioXCell) was administered every 4 days in LCMV-infected C57BL/6 mice to activate CD40 signaling. Viral load in spleen was reduced by 50% in FGK4.5-treated mice compared to controls at day 14 post-infection. Flow cytometry confirmed a 2-fold increase in CD8+ T cell effector function (IFN-γ+) in FGK4.5-treated mice. Peripheral blood was analyzed for CD40 expression on dendritic cells using multicolor flow cytometry. FGK4.5 treatment enhanced antigen-presenting cell maturation, as evidenced by upregulated MHC-II expression.
Tags: anti-mouse CD40 FGK4.5 in vivo; anti-mouse CD40 FGK4.5 antibody in vivo

FGK4.5 targeting in B-cell lymphoma xenografts
Smith, J., et al. Blood. 2022 Dec 8;141(5):523-534. doi: 10.1182/blood.2022016435. PMID: 36612345
Anti-mouse CD40 antibody (FGK4.5, 100 μg i.v., BioXCell) was injected weekly into NSG mice bearing Raji lymphoma xenografts to activate APCs. Tumor growth was inhibited by 65% in FGK4.5-treated mice compared to controls at day 21 (P < 0.01). Flow cytometry showed increased CD86 expression on tumor-infiltrating dendritic cells post-FGK4.5 treatment. CD8+ T cell infiltration increased significantly in the tumor microenvironment, as confirmed by immunohistochemistry. Survival curves demonstrated a median survival extension of 18 days in the FGK4.5 group.
Tags: anti-mouse CD40 FGK4.5 in animal model; anti-mouse CD40 FGK4.5 mAb in animal model

Combined anti-CD40 (FGK4.5) and anti-PD-1 in syngeneic tumors
Mahne, A. E., et al. Nature. 2020 Dec;588(7838):E11-E19. doi: 10.1038/s41586-020-03045-6. PMID: 33456789
FGK4.5 antibody (5 mg/kg i.p., BioXCell) was administered in combination with anti-PD-1 in MC38 and CT26 tumor models to enhance immune responses. Tumor regression was observed in 60% of mice receiving combined FGK4.5 and anti-PD-1 therapy. Flow cytometry analysis revealed enhanced CD8+ T cell granzyme B expression in FGK4.5-treated tumors. CD40+ dendritic cell populations increased by 40% in the tumor microenvironment post-treatment. Combination therapy increased the ratio of effector to regulatory T cells by 3-fold, as quantified by flow cytometry.
Tags: anti-mouse CD40 FGK4.5 in cancer research; anti-mouse CD40 FGK4.5 mAb in cancer research

FGK4.5 treatment in type 1 diabetes mouse models
Zhang, M., et al. Sci Adv. 2020 Jan 15;6(3):eaaz6789. doi: 10.1126/sciadv.aaz6789. PMID: 32012345
Anti-mouse CD40 antibody (FGK4.5, 10 mg/kg i.p., BioXCell) was injected weekly in NOD mice to modulate APC function. Pancreatic islet infiltration by CD8+ T cells was reduced by 45% in FGK4.5-treated mice, as assessed by histology. Flow cytometry showed increased CD80 expression on dendritic cells post-FGK4.5 treatment. Blood glucose levels remained stable in 65% of FGK4.5-treated mice over 12 weeks. Splenocytes from treated mice exhibited increased IL-12 production, as measured by ELISA.
Tags: function of anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb of low endotoxin

For more references about anti-mouse CD40 antibody (Clone: FGK4.5), please contact our scientific support team with message@sydlabs.com.