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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls and Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2a Monoclonal Antibody.
Clone: FGK4.5 / FGK45.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: FGK4.5) specifically binds to the mouse CD40 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD40 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: FGK4.5 / FGK45.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: FGK4.5) specifically binds to the mouse CD40 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD40 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The FGK4.5 antibody binds to the cell-surface molecule CD40, a member of the tumor necrosis factor receptor superfamily. CD40 is expressed by antigen-presenting cells (APC) and many tumor cells.
Related Recombinant IgG Reference Antibodies:
Recombinant rat lgG2a isotype control antibody
Recombinant mouse IgG2a isotype control antibody
1、DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance
Patricia Conde, et al. Immunity. 2015 Jun 16;42(6):1143-58. doi: 10.1016/j.immuni.2015.05.009. Epub 2015 Jun 9. PMID: 26070485
Agonistic anti-CD40 mAb (clone FGK4.5 mAb) was produced by ... CD40-mediated priming independent of CD40L was achieved by intravenous (i.v.) injection of 100 μg of agonistic anti-CD40 mAb on days 0 and +1 relative to transplantation (Gorbachev and Fairchild, 2004). Blocking antibody to IFN-γ (Clone R4-6A2) was produced by BioXcell. Anti-IFN-γ mAb was injected at 500 μg on days 0 and +1 relative to transplantation. Blocking antibody to CSF1 (clone 5A1) (Lokeshwar and Lin, 1988) and CSF1R (clone AFS98) (Sudo et al., 1995) were produced by BioXcell. Anti-CSF1 mAb was injected at 150 μg i.p. on days −1, +1, +2, +3, and +4 relative to transplantation, which is known to neutralize the biological functions of CSF1 in vivo (Gregory et al., 1992).
Tags: anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb
2、DC-SIGN(+) LAG3+ Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1+ Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis
David Bauché, et al. Immunity. 2018 Aug 21;49(2):342-352.e5. doi: 10.1016/j.immuni.2018.07.007. Epub 2018 Aug 7. PMID: 30097293
C57BL/6 Rag2-/- and Foxp3GDL mice were used at 8-16 weeks of age. At day 0, mice were administered i.p with 100 μg of anti-CD40 (IgG2a, monoclonal Ab FGK45) to induce IBD (Rolink et al., 1996). The naïve group did not receive any treatment. Weight loss was measured relative to initial weight in all studies.
Tags: anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb
3、Unique potential of 4-1BB agonist antibody to promote durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine
The following agonistic and antagonistic antibodies to various T-cell costimulatory modulating receptors were purchased from BioXcell at <1 endotoxin unit/mg of LPS and tested with and without the E6/E7 peptide vaccine: antibody to CTLA-4 (9H10 at 100 μg per dose), to PD-1 (RMP1-14 at 250 μg per dose), to OX-40 (OX-86 at 100 μg per dose), to GITR (DTA-1 at 350 μg per dose), to 4–1BB (LOB12.3 at 350 μg per dose) and to CD40 (FGK4.5 at 100 μg per dose).
Tags: anti-mouse CD40 FGK4.5 in vivo; anti-mouse CD40 FGK4.5 antibody in vivo
For more references about anti-mouse CD40 antibody (Clone: FGK4.5) please contact our scientific support team with message@sydlabs.com.
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