BP000027-CYT-336: Recombinant Mouse Vascular Endothelial Growth Factor (VEGF) Protein
Source: E. coli-derived.
The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml corresponding to a specific activity of 0.2-1 MIU/mg.
Purity: > 95.0% as determined by RP-HPLC and SDS-PAGE.BP000027-CYT-577: Recombinant Human Vascular Endothelial Growth Factor (VEGF) Protein, Yeast
Source: Pichia Pastoris-derived.
Determined by its ability to stimulate 3H-Thymidine incorporation in human umbilical vein endothelial cells, the ED50 for this effect was found to be 2-6 ng/ml.
Purity: > 90% by SDS-PAGE.BP000027-CYT-619: Recombinant Human VEGF (121 a.a) Protein, His Tag
Source: E. coli-derived.
The ED50 for this effect is <4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell, corresponding to a specific activity of less than 238,095 units/mg.
Purity: > 95% by SDS-PAGE.BP000027-CYT-680: Recombinant Mouse Vascular Endothelial Growth Factor (VEGF) Protein, His Tag
Source: E. coli-derived.
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.
Purity: >90% by SDS-PAGE.BP005727: Biotinylated Human VEGF121 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF R1 protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human VEGF121 protein with the C-His tag with the EC50 of 16.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005778: Human VEGF165 Protein with No Tag
Source: The recombinant Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF165 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGFR2, mFc tag with the EC50 of 42.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005436: Human VEGF165 Protein with the C-His-Avi Tag
Source: The recombinant Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF165 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-VEGF165 Antibody protein with the C-hFc tag with the EC50 of 4.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005474: Biotinylated Human VEGF165 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-VEGF165 Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human VEGF165 protein with the C-His tag with the EC50 of 11.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003503: Human VEGF121 Protein with No Tag
Source: The recombinant Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF121, No tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGF R1, His tag with the EC50 of 21.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP004957: Human VEGF121 Protein with the C-His-Avi Tag
Source: The recombinant Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF121 protein with the C-His-Avi tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human VEGFR1 protein with the C-His tag with the EC50 of 42 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000027-CYT-200: Recombinant Human VEGF (121 a.a.) Protein, Sf9
Source: Sf9, Insect Cells-derived.
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-5 ug/ml corresponding to a Specific Activity of 200,000-500,000 IU/mg.
Purity: >90% by SDS-PAGE and RP-HPLC.BP000027-CYT-225: Recombinant Human Vascular Endothelial Growth Factor (VEGF) Protein, HEK
Source: HEK 293 (Human Embryonic Kidney cell line)-derived.
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-6 ug/ml corresponding to a Specific Activity of 166,667-1,000,000 IU/mg.
Purity: > 95% by SDS-PAGE.