BP005727: Biotinylated Human VEGF121 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF R1 protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human VEGF121 protein with the C-His tag with the EC50 of 16.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005474: Biotinylated Human VEGF165 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-VEGF165 Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human VEGF165 protein with the C-His tag with the EC50 of 11.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003503: Human VEGF121 Protein with No Tag

Source: The recombinant Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF121, No tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGF R1, His tag with the EC50 of 21.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004957: Human VEGF121 Protein with the C-His-Avi Tag

Source: The recombinant Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF121 protein with the C-His-Avi tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human VEGFR1 protein with the C-His tag with the EC50 of 42 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005778: Human VEGF165 Protein with No Tag

Source: The recombinant Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF165 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGFR2, mFc tag with the EC50 of 42.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005436: Human VEGF165 Protein with the C-His-Avi Tag

Source: The recombinant Human VEGF165 Protein, (amino acids Ala27-Arg191) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF165 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-VEGF165 Antibody protein with the C-hFc tag with the EC50 of 4.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP000027-CYT-200: Recombinant Human VEGF (121 a.a.) Protein, Sf9

Source: Sf9, Insect Cells-derived.
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-5 ug/ml corresponding to a Specific Activity of 200,000-500,000 IU/mg.
Purity: >90% by SDS-PAGE and RP-HPLC.

BP000027-CYT-225: Recombinant Human Vascular Endothelial Growth Factor (VEGF) Protein, HEK

Source: HEK 293 (Human Embryonic Kidney cell line)-derived.
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-6 ug/ml corresponding to a Specific Activity of 166,667-1,000,000 IU/mg.
Purity: > 95% by SDS-PAGE.

BP000027-CYT-226: Recombinant Mouse Vascular Endothelial Growth Factor (VEGF) Protein, Sf9

Source: Baculovirus Sf9 cells-derived.
The ED50 range, determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (measured by 3H-thymidine uptake) is 1-2 ug/ml, corresponding to a specific activity of 1x106 Units/mg.
Purity: > 95% by SDS-PAGE and RP-HPLC.

BP000027-CYT-241: Recombinant Human Vascular Endothelial Growth Factor (VEGF) Protein

Source: E. coli-derived.
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-8 ug/ml corresponding to a Specific Activity of 125,000-1,000,000 IU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.

BP000027-CYT-260: Recombinant Human Vascular Endothelial Growth Factor (VEGF) Protein, CHO

Source: Chinese Hamster Ovarian Cell-derived.
Determined by its ability to stimulate 3H-Thymidine incorporation in human ublical vein endothelial cells, the ED50 for this effect was found to be 2-6 ng/ml corresponding to a Specific Activity of 166,667-500,000 IU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.

BP000027-CYT-336: Recombinant Mouse Vascular Endothelial Growth Factor (VEGF) Protein

Source: E. coli-derived.
The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml corresponding to a specific activity of 0.2-1 MIU/mg.
Purity: > 95.0% as determined by RP-HPLC and SDS-PAGE.