BP005723: Biotinylated Cynomolgus/Rhesus macaque FcRn Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Cynomolgus/Rhesus macaque FcRn Protein, (amino acids Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005025: Biotinylated Human FcRn Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human FcRn Protein, (amino acids Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003192: Biotinylated Mouse FcRn Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Mouse FcRn Protein, (amino acids Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 1.22 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004385: Cynomolgus/Rhesus macaque FcRn Protein with the C-His Tag
Source: The recombinant Cynomolgus/Rhesus macaque FcRn Protein, (amino acids Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus/Rhesus macaque FcRn protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-FcRn Antibody protein with the C-hFc tag with the EC50 of 6.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.
BP4095: Recombinant human FcRn protein
Source: HEK 293 cell-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.BP004299: Human FcRn Protein with the C-His Tag
Source: The recombinant Human FcRn Protein, (amino acids Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human FcRn protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-FcRn Antibody protein with the C-hFc tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004300: Mouse FcRn Protein with the C-His Tag
Source: The recombinant Mouse FcRn Protein, (amino acids Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.