BP005445: Human Fc gamma RI/CD64 Protein with the C-His-Avi Tag
Source: The recombinant Human Fc gamma RI/CD64 Protein, (amino acids Gln16-Pro288) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 1.94 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004378: Biotinylated Human Fc gamma RI/CD64 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Fc gamma RI/CD64 Protein, (amino acids Gln16-Pro288) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004497: Mouse Fc gamma RIV/CD16-2 Protein with the C-His Tag
Source: The recombinant Mouse Fc gamma RIV/CD16-2 Protein, (amino acids Gly21-Gln203) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 146 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.
BP005614: Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein, (amino acids Ala36-Ile218(R167)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 1.03 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP4096: Recombinant human Fc gamma RIIA protein
Source: HEK 293 cell-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant human Fc gamma RIIA protein binds to human IgG as a low affinity receptor.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.BP005404: Human Fc gamma RIIIB/CD16b (NA2) Protein with the C-His-Avi Tag
Source: The recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein, (amino acids Gly17-Ser200(NA2)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.52 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP4097: Recombinant human Fc gamma RIIB/C protein
Source: HEK 293 cell-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant human Fc gamma RIIB/C protein binds to human IgG as a low affinity receptor.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.