BP005260: Human IL-27RA/TCCR Protein with the C-hFc Tag
Source: The recombinant Human IL-27RA/TCCR Protein, (amino acids Gly34-Lys516) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-27 RA protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-27 protein with the C-His tag with the EC50 of 55.9 ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.