BP004279: Biotinylated Human IL-18 Protein with No Tag
Source: The recombinant Biotinylated Human IL-18 Protein, (amino acids Tyr37-Asp193) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-18BP, hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-18, No tag with the EC50 of 5.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005246: Human IL-1 Beta/IL-1F2 Protein with the C-His-Avi Tag
Source: The recombinant Human IL-1 Beta/IL-1F2 Protein, (amino acids Ala117-Ser269) fused with a His-Avi tag at the C-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-1 Beta protein with the C-His-Avi tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-1R2 protein with the C-hFc tag with the EC50 of 0.12 ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004932: Biotinylated Human IL-1 Beta/IL-1F2 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein, (amino acids Ala117-Ser269) fused with a His-Avi tag at the C-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-1R2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta protein with the C-His tag with the EC50 of 13.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.