BP005022: Biotinylated Human GFRAL/GFR alpha-like Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human GFRAL/GFR alpha-like Protein, (amino acids Ser19-Glu351) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GDF15 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GFRAL protein with the C-His tag with the EC50 of 89.1 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005603: Mouse GFRAL/GFR alpha-like Protein with the N-His-Avi Tag
Source: The recombinant Mouse GFRAL/GFR alpha-like Protein, (amino acids Gln20-Glu350) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse GFRAL protein with the N-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse GDF15 protein with the N-hFc tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.