BP005444: Human Fc gamma RIIIA/CD16a (F176) Protein with the C-His-Avi Tag
Source: The recombinant Human Fc gamma RIIIA/CD16a (F176) Protein, (amino acids Gly17-Gln208(F176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Fc gamma RIIIA (F176) protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Fc gamma RIIIA Antibody protein with the C-hFc tag with the EC50 of 4.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.92 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005710: Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein, (amino acids Gly17-Gln208(F176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-Fc gamma RIIIA Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human Fc gamma RIIIA (F176) protein with the C-His-Avi tag with the EC50 of 35.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.