BP005071: Human/Cynomolgus/Rhesus macaque CD28 Protein with the N-hFc Tag
Source: The recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein, (amino acids Asn19-Pro152) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human/Cynomolgus/Rhesus macaque CD28 protein with the N-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD28 Antibody protein with the N-hFc tag with the EC50 of 46.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004425: Human/Cynomolgus/Rhesus macaque CD28 Protein with the N-mFc Tag
Source: The recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein, (amino acids Asn19-Pro152) fused with a mFc tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human/Cynomolgus/Rhesus macaque CD28 protein with the N-mFc(IgG2a) tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CD28 Antibody protein with the N-hFc tag with the EC50 14.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005365: Human/Cynomolgus/Rhesus macaque CD28 Protein with the N-His-Avi Tag
Source: The recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein, (amino acids Asn19-Pro152) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human/Cynomolgus/Rhesus macaque CD28 protein with the N-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody protein with the N-hFc tag with the EC50 of 18.4 ng/ml determined by ELISA (QC Test). The affinity constant of 5.15 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.