Anti-Mouse VEGFR-2 Monoclonal Antibodies

Background of Anti-Mouse VEGFR-2 Monoclonal Antibody

Our recombinant VEGFR-2 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse VEGFR-2 monoclonal antibody (hybridoma clone name or number: DC101).

PA007527.r1: In Vivo Grade Recombinant Anti-mouse VEGFR-2 Monoclonal Antibody (Clone: DC101), Rat IgG1 Kappa

The in vivo grade recombinant anti-mouse VEGFR-2 rat IgG1 monoclonal antibody was produced in mammalian cells.
Specificity: VEGFR-2.
Clone: DC101.
Isotype: rat IgG1, kappa.
Applications: Western blot, immunoprecipitation (IP), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse VEGFR-2 antibodies (clone of DC101) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Syd Labs provides the following anti-mouse PD1 (PD-L1) and VEGFR2 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD 1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
In vivo grade recombinant anti-mouse VEGFR2 monoclonal antibodies (Clone DC101)

Syd Labs provides the following anti-mouse PD-1 / VEGFR-2 bispecific antibodies:
In vivo grade recombinant anti-mouse PD 1 / VEGFR2 bispecific antibodies (Clone RMP1-14.1 / DC101)
In vivo grade recombinant anti-mouse PD-1 / VEGFR-2 bispecific antibodies (Clone 29F.1A12.1 / DC101)

Syd Labs provides the following anti-mouse PD-L1 / VEGFR-2 bispecific antibodies:
In vivo grade recombinant anti-mouse PDL1 / VEGFR-2 bispecific antibodies (Clone 10F.9G2.1 / DC101)

References of Anti-mouse VEGFR-2 Monoclonal Antibody:

Distinct functions of epidermal and myeloid-derived VEGF-A in skin tumorigenesis mediated by HPV8
Ding, X., et al. Cancer Res. 2015 Jan 15;75(2):330-43. doi: 10.1158/0008-5472.CAN-13-3007. PMID: 25414138
VEGF mediates its activities primarily through binding to two receptor tyrosine kinases, known as VEGFR1 and VEGFR2, as well as to coreceptors including neuropilins. ...Inhibition of VEGFR2 was performed by intraperitoneal injections of anti-VEGFR2 blocking antibody (DC101, BioXcell) or the corresponding isotype-matched control antibody (IgG1, BioXcell; 40 mg/kg body weight in PBS). ...This antibody has been shown to be a potent inhibitor of VEGFR2. ...To investigate whether the vasculature is a target of VEGF in HPV8-mediated skin tumor development, we used an antibody-based strategy to inhibit angiogenesis by blocking VEGFR2 signaling in endothelial cells. ...For this purpose at days 10 and 34 after UV exposure, the epidermis was separated from dermis and epidermal expression for VEGF, VEGFR2, VEGFR1, and Nrp1 was determined by qRT-PCR analysis.
Tags: anti-mouse VEGFR-2 DC101; anti-mouse VEGFR-2 DC101 antibody

Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process
Kizhatil, K., et al. PLoS Biol. 2014 Jul;12(7):e1001912. doi: 10.1371/journal.pbio.1001912. PMID: 25051267
Functional inhibition of KDR (VEGFR2), a critical receptor in initiating angiogenesis, shows that this receptor is required during canalogenesis. ...FLT4 (VEGFR3) is another key protein required for lymphatic development and also serves as a marker for lymphatics. ...To begin to define the molecules necessary for SC development, we tested the functional importance of the tyrosine kinase receptor KDR (alias VEGFR2, a receptor for vascular endothelial growth factor, or VEGF). ...This is consistent with a recent report that mice heterozygous for mutations in both Kdr and Flt1 (Vegfr1) have elevated IOP. ...The DC101- and control-injected eyes have a similar LVP. Note that there is substantial regional variation in LVP architecture in every eye.
Tags: anti-mouse VEGFR-2 DC101 antibody in vivo; anti-mouse VEGFR-2 DC101 in animal model

Junctional adhesion molecule-A regulates vascular endothelial growth factor receptor-2 signaling-dependent mouse corneal wound healing
Chatterjee, S., et al. PLoS One. 2013 May 8;8(5):e63674. doi: 10.1371/journal.pone.0063674. PMID: 23667656
VEGF-A signals by binding to its receptors VEGFR-1 (FLT-1) and VEGFR-2 and is known to also promote inflammation and epithelial to mesenchymal transition. ...VEGF-A signaling through its receptors FLT-1 and VEGFR-2 also regulates TGF? expression that in turn regulates corneal wound healing. ...This upregulation of VEGF-A was found to be functionally relevant since treatment of Jam-A deficient mice with a function blocking VEGFR-2 antibody resulted in a partial rescue of the observed wound-healing defect. ...Further, the mRNA levels of FLT1 (VEGFR-1) or VEGFR-2 appeared to be slightly increased in Jam-Agt/gt compared to WT mice although these differences were not statistically significant. ...The above results show that JAM-A negatively regulates wound-induced inflammation, angiogenesis and scarring by modulating the VEGF-A/VEGFR-2 pathway.
Tags: anti-mouse VEGFR-2 DC101 antibody in animal model; anti-mouse VEGFR-2 DC101 in cancer research

Inhibition of VEGFR-2 reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function
Villalta, S. A., et al. Diabetes. 2013 Aug;62(8). doi: 10.2337/db12-1619. PMID: 23835340
We found that RTK inhibitors (RTKIs) and VEGF or VEGFR-2 antibodies reversed diabetes when administered at the onset of hyperglycemia. ...Finally, examination of human pancreata from patients with T1D revealed that VEGFR-2 was confined to the islet vascularity, which was increased in inflamed islets. ...VEGF regulates vasculogenesis and angiogenesis largely through activation of VEGFR-2. ...The phenotypes of VEGFR-1 and VEGFR-2?null mice indicate that, although VEGF-A has limited function through VEGFR-1, the vascular remodeling functions of VEGF-A are largely mediated through the activation of VEGFR-2. ...In this study, we determined whether VEGFR-2 might be involved in the pathogenesis of T1D and tested the therapeutic efficacy of VEGFR-2 inhibition in the NOD mouse model of T1D.
Tags: anti-mouse VEGFR-2 DC101 in mouse tumor model; function of anti-mouse VEGFR-2 DC101

Global lymphoid tissue remodeling during a viral infection is orchestrated by a B cell-lymphotoxin-dependent pathway
Kumar, V., et al. Blood. 2010 Jun 10;115(23). doi: 10.1182/blood-2009-10-250118. PMID: 20185585
Quantitative polymerase chain reaction (qPCR) of cDNA isolated from control and LCMV-infected PLNs was carried out with mouse VEGF-A and VEGFR2 primers by the use of LightCycler FastStart DNA master SYBR Green I kit (Roche Diagnostics) on a LightCycler 1.5 (Roche Diagnostics) as described. ...Anti-VEGFR2 (clone DC101; BioXcell) was injected on day 0 and 3 after infection (2 × 500 ?g/mouse). ...The VEGFR/PDGFR inhibitor sunitinib (Selleck) was used at 35 mg/kg in citrate-buffered water and administered daily by oral gavage for 7 days. ...We next performed LCMV infections in the presence of blocking Abs against VEGFR-2 or VEGF-A or in presence of the VEGFR/PDGFR inhibitor sunitinib. ...(C) Inguinal LN volume on day 0 (D0) or day 8 (D8) after infection after treatment of mice with control Ig or anti-VEGFR2, anti?VEGF-A Abs, or sunitinib. Examples were pooled from 1 to 2 independent experiments with 3 to 5 mice per treatment.
Tags: bioactivity of anti-mouse VEGFR-2 DC101; anti-mouse VEGFR-2 DC101 antibody of low endotoxin

For more references about Anti-mouse VEGFR-2 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.