PA007712.m2a: In Vivo Grade Recombinant Anti-mouse V beta 8 TCR Monoclonal Antibody (Clone: F23.1), Mouse IgG2a Kappa
Recombinant mouse IgG2a monoclonal antibody.
Clone: F23.1.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse V beta 8 TCR monoclonal antibody (clone: F23.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: F23.1) specifically binds to mouse V beta 8 TCR.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse V beta 8 TCR protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse V beta 8 TCR monoclonal antibody of clone F23.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Bothur, E., et al.
Nat Commun. 2015 Oct 13.
PMID: 26815406
The superantigen SEB is recognized by all TCRs carrying a Vβ gene of the Vβ8 family, but not by Vβ6 family members. ...As shown in Fig. 1f, Vβ8+ and Vβ6+ iTregs kept high levels of FOXP3 after re-culture without SEB, while in its presence Vβ8+ but not Vβ6+ iTregs downregulated FOXP3 to a great extent. ...Cells re-stimulated by SEB were first incubated for 5 min with the αCD16/32 FcR blocking antibody, followed by extracellular staining using either αVβ6 plus α-rat-IgG-FITC or αVβ8 (F23.1; ascites, 1:100) plus α-mouse-IgG-Alexa488.
(Tags: activity of Anti-Mouse V beta 8 TCR Monoclonal; Anti-Mouse V beta 8 TCR Monoclonal)
Bell, S. J., et al.
Cell Immunol. 1995 Jan;160(1):58-64.
PMID: 7842486
Primary in vivo challenge with the superantigen staphylococcal enterotoxin B (SEB) induces polyclonal proliferation of an unusually large proportion of circulating T-cells that bear the Vβ8 TCR domain. ...Recently we demonstrated that more cells than just Vβ8+ T-cells undergo clonal proliferation after challenge with SEB. ...These findings suggested that non-Vβ8+ T-cells may have a role in the induction of superantigen-induced anergy. ...Results indicated that challenge of SWM-primed mice with SEB abrogated the Vβ8-associated SWM-specific T-cell memory for an extended but transient period of time.
(Tags: Anti-Mouse V beta 8 TCR Monoclonal antibody for animal model; Anti-Mouse V beta 8 TCR Monoclonal antibody for flow cytometry)
Aimé-Sempé, C., et al.
J Neuroimmunol. 1995 May;58(2):191-200.
PMID: 7759608
The specific proliferation towards AChR was found to be blocked by anti-Vβ8.1,2,3 and to a lesser extent by anti-Vβ5 mAbs, but not by the other antibodies used (anti-Vβ2, Vβ6, Vβ9). ...Involvement of Vβ8 subfamilies was also explored in vivo. ...After 7 days of treatment, there was a striking inhibition of the proliferative response of cells from anti-Vβ8.1,2,3-treated mice and a moderate inhibition when using anti-Vβ8.1,2 and anti-Vβ8.2 antibodies. ...Monoclonal antibody F23.1 against T cell bearing Vβ8 TCR elements can prevent the development of insulitis in NOD mice.
(Tags: Anti-Mouse V beta 8 TCR Monoclonal antibody in vivo; Anti-Mouse V beta 8 TCR Monoclonal for mouse tumor model)
Dieli, F., et al.
Immunology. 1998 Apr;93(4):469-77.
PMID: 9659217
Analysis of the role played by Vβ8+ T lymphocytes during in vivo responses to TNCB, have been undertaken using TNCB-specific T-cell lines. ...Against this background, we decided to investigate the role played by Vβ8+ T lymphocytes in two examples of immune response to TNCB in vivo, namely the CS reaction and its passive transfer. ...To confirm the requirement for Vβ8+ lymphocytes in the CS reaction to TNCB, passive transfer experiments were undertaken. ...To ascertain which cell within the Vβ8+ population was important in the passive transfer of the CS reaction, the Vβ8+ population was further depleted of γδ+ or αβ+ cells and cotransferred together with Vβ8+ cells into naive recipient mice.
(Tags: function of Anti-Mouse V beta 8 TCR Monoclonal; rat Anti-Mouse V beta 8 TCR Monoclonal)