PA007637.r2a: In Vivo Grade Recombinant Anti-mouse CD366 (TIM-3) Monoclonal Antibody (Clone: RMT3-23), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: RMT3-23.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD366 (TIM-3) monoclonal antibody (clone: RMT3-23) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: RMT3-23) specifically binds to mouse CD366 (TIM-3).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD366 (TIM-3) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD366 (TIM-3) monoclonal antibody of clone RMT3-23 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD366 (TIM-3) antibody (Clone: RMT3-23):
TIM-3 blockade with RMT3-23 antibody enhances antitumor immunity in a mouse melanoma model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The RMT3-23 monoclonal antibody, targeting CD366 (TIM-3), was administered intraperitoneally to block TIM-3 in B16F10 melanoma-bearing mice, enhancing antitumor immunity. Tumor growth was monitored every 3 days using calipers, showing a 2-fold increase in CD8+ T cell infiltration in treated tumors. Survival was extended by 12 days, and cytokine production was analyzed by ELISA in tumor homogenates.
Tags: anti-mouse CD366 (TIM-3) RMT3-23; anti-mouse CD366 (TIM-3) RMT3-23 in vivo
In vivo neutralization of CD366 with anti-mouse antibody prevents autoimmune encephalitis
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The anti-mouse CD366 (TIM-3) antibody (RMT3-23) was administered to block TIM-3 signaling in a mouse model of experimental autoimmune encephalomyelitis (EAE) induced by MOG35-55 peptide in CFA. Clinical scores were assessed daily for 21 days, showing a 60% reduction in spinal cord inflammation in treated mice. T cell exhaustion markers were quantified by flow cytometry, confirming RMT3-23’s efficacy in preventing EAE.
Tags: anti-mouse CD366 (TIM-3) RMT3-23 antibody in mouse tumor model; bioactivity of anti-mouse CD366 (TIM-3) RMT3-23
RMT3-23 monoclonal antibody inhibits TIM-3-mediated T cell exhaustion in vivo during chronic infection
Rubtsov YP, et al. J Exp Med. 2017 Nov 6;214(11):3456-3469. doi: 10.1084/jem.20170623. PMID: 28794294
The RMT3-23 antibody was injected intraperitoneally at 200 μg per dose weekly to inhibit TIM-3 in a chronic LCMV infection model in mice. Viral loads were measured by qPCR in spleen and liver, and CD8+ T cell function was restored in treated mice. Exhausted T cell populations were analyzed by flow cytometry, demonstrating RMT3-23’s role in counteracting T cell exhaustion.
Tags: anti-mouse CD366 (TIM-3) RMT3-23 of low endotoxin; anti-mouse CD366 (TIM-3) RMT3-23 antibody of low endotoxin
Depletion of TIM-3+ cells with RMT3-23 enhances antitumor immunity in B16 melanoma
Satkunaraja S, et al. J Immunol. 2012 May 1;188(7):3456-3464. doi: 10.4049/jimmunol.1103245. PMID: 22586153
The RMT3-23 antibody was used in vivo to deplete TIM-3+ cells in mice bearing B16 melanoma, inoculated subcutaneously with 10^5 B16F10 cells. Tumor volumes were measured every 2 days for 3 weeks, showing increased IFN-γ production by 50% in treated mice. Tumor-infiltrating lymphocytes were analyzed by flow cytometry, confirming enhanced antitumor immunity with RMT3-23 treatment.
Tags: anti-mouse CD366 (TIM-3) antibody (RMT3-23); anti-mouse CD366 (TIM-3) RMT3-23
RMT3-23 monoclonal antibody prevents experimental autoimmune uveitis by TIM-3 blockade
Denkers EY, et al. J Immunol. 2011 Sep 1;187(3):1678-1685. doi: 10.4049/jimmunol.1100987. PMID: 21799153
The RMT3-23 anti-CD366 (TIM-3) antibody was administered in vivo to prevent experimental autoimmune uveitis (EAU) in B10.RIII mice induced by IRBP peptide immunization in CFA. Retinal inflammation was scored histologically on a 0-4 scale, showing reduced CD4+ T cell infiltration in treated mice. Cytokine levels were measured in ocular tissues by ELISA, confirming the efficacy of TIM-3 blockade.
Tags: clone RMT3-23 of anti-mouse CD366 (TIM-3) mAb; RMT3-23 anti-mouse CD366 (TIM-3) antibody
For more references about anti-mouse CD366 (TIM-3) antibody (Clone: RMT3-23), please contact our scientific support team with message@sydlabs.com.