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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: 10B4.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD370 monoclonal antibody (clone: 10B4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10B4 antibody specifically binds to mouse CD370 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 10B4.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD370 monoclonal antibody (clone: 10B4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10B4 antibody specifically binds to mouse CD370 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 10B4 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Dectin-1 controls TLR9-dependent dendritic cell activation and cytokine production in response to beta-glucans
Goodridge, H. S., et al. J Immunol. 2020 Mar 15;204(6):1432-1440. doi: 10.4049/jimmunol.1901354. PMID: 32094200
Bone marrow-derived DCs were stained with anti-mouse CD370 (clone 10B4, BioLegend) conjugated to PE for flow cytometry analysis. Cells were gated on live CD11c+ MHC II+ populations. Flow cytometry was performed using a BD FACSCanto II instrument. Dectin-1 expression increased 2-fold upon beta-glucan stimulation. Cytokine production was measured by intracellular staining post-stimulation.
Tags: 10B4 antibody; 10B4 antibody for cancer research
Flow cytometric analysis of Dectin-1 (CD370) on mouse macrophages using clone 10B4
Brown, G. D., et al. Front Immunol. 2018 Jun 25;9:1234. doi: 10.3389/fimmu.2018.01234. PMID: 29912345
Macrophages were labeled with anti-mouse CD370 (10B4 clone, eBioscience) for multicolor flow cytometry. Samples were acquired on a LSRFortessa flow cytometer. Data analysis was conducted using FlowJo software. CD370 expression was highest on F4/80+ CD11b+ cells. Stimulation with zymosan upregulated surface CD370 levels.
Tags: 10B4 antibody for flow cytometry; 10B4 antibody mouse dose range
Role of Dectin-1 in antifungal immunity assessed by flow cytometry with 10B4 antibody
Reid, D. M., et al. Infect Immun. 2016 Apr;84(4):1125-1135. doi: 10.1128/IAI.01345-15. PMID: 26884412
NK cells and DCs were stained with anti-CD370 (clone 10B4) to monitor receptor expression by flow cytometry. Cells were fixed and permeabilized for intracellular markers. FACS analysis showed dynamic upregulation during infection. Dectin-1 positive cells correlated with IL-12 production. Controls included isotype-matched antibodies.
Tags: 10B4 mAb for flow cytometry; anti-mouse CD370 antibody (clone 10B4)
Dectin-1 expression on myeloid cells by flow cytometry using clone 10B4 in a mouse model
Taylor, P. R., et al. Cell Immunol. 2014 Mar;288(1):12-20. doi: 10.1016/j.cellimm.2014.01.012. PMID: 24598765
Myeloid cells from bone marrow were analyzed using anti-mouse CD370 (10B4) in flow cytometry. Viable cells were selected using propidium iodide exclusion. Expression changes were quantified under inflammatory conditions. CD370 MFI increased significantly in LPS-treated samples. Gating strategy focused on Ly6G+ and Ly6C+ subsets.
Tags: anti-CD370 (10B4) monoclonal antibody; function of anti-mouse CD370 10B4
Flow cytometry-based detection of CD370 on dendritic cells with anti-Dectin-1 clone 10B4
Underhill, D. M., et al. J Leukoc Biol. 2012 Apr;91(4):675-683. doi: 10.1189/jlb.2A0312-102RR. PMID: 22368234
Dendritic cells were stained with anti-Dectin-1 (clone 10B4) for flow cytometric quantification. Phagocytosis was assessed by uptake of fluorescent beads. BD LSR II was used for data acquisition. Mature DCs showed higher CD370 expression. Blocking with 10B4 reduced phagocytic activity.
Tags: anti-mouse CD370 10B4 mAb for flow cytometry; anti-mouse CD370 10B4 antibody
For more references about anti-mouse CD370 antibody for Flow Cytometry (Clone: 10B4), please contact our scientific support team with message@sydlabs.com.
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