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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: 10B4.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD370 monoclonal antibody (clone: 10B4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10B4 antibody specifically binds to mouse CD370 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 10B4.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD370 monoclonal antibody (clone: 10B4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10B4 antibody specifically binds to mouse CD370 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 10B4.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD370 monoclonal antibody (clone: 10B4) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 10B4 antibody specifically binds to mouse CD370 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 10B4 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
1、New insight into the Nox4 subcellular localization in HEK293 cells: first monoclonal antibodies against Nox4.
Nguyen, M. V. C., et al. Biochimie. 2011 Mar;93(3):457-68. doi: 10.1016/j.biochi.2010.11.001. PMID: 21075166
In order to investigate subcellular localization and topology of Nox4, 5 novel monoclonal antibodies, named 5F9, 6B11, 8E9, 10B4, 7C9, were developed. ...The other 2 mAbs 10B4 and 7C9 were shown to have a very weak reactivity after purification. ...Five monoclonal antibodies specific to Nox4 were raised for the first time and characterized in the present work. ...The current findings focus on distinct properties of these mAbs referring to identification and subcellular localization of Nox4 as NADPH oxidase activity. ...But understanding of the role of Nox4 in the production of ROS and of regulation mechanism of oxidase activity is largely unknown.
Tags: 10B4 antibody; 10B4 antibody for cancer research
2、On the mechanisms of human T cell proliferation induced by the 10B4 molecule.
Katayama, K., et al. Hokkaido Igaku Zasshi. 1987 Dec;62(6):866-72. PMID: 3127316
The 10B4 antibody together with goat anti-mouse immunoglobulin antibody induced significant increase of DNA and RNA syntheses in T cells with peak responses on day 9 and on day 7, respectively. ...The 10B4 system of human T cells seems to constitute a member of T cell receptor complex. ...We have analyzed the mechanisms by which a monoclonal antibody against the 10B4 molecule activates human peripheral T cells. ...This activation process is mediated by interleukin 2 (IL-2) and its receptor (IL-2R), because (1) IL-2 activity was detected in the culture supernatants, (2) the percentage of IL-2R positive cells increased during the culture period, with a peak on day 9, and (3) the 10B4-induced T cell proliferation was inhibited by anti IL-2R antibody. Blocking studies with pharmacological agents showed that in the 10B4-induced system, a protein kinase C (PK-C) inhibitor, palmitoylcarnitine, blocked DNA synthesis, RNA synthesis, IL-2 production and IL-2R expression whereas a Ca ion channel blocker, verapamil, inhibited DNA synthesis, RNA synthesis, IL-2 production but not IL-2R expression. ...It is thus concluded that PK-C activation is required for IL-2 production and IL-2R expression and that channel-mediated Ca ion influx is important for IL-2 production but may not be needed for IL-2R expression.
Tags: 10B4 antibody for flow cytometry; 10B4 antibody mouse dose range
For more references about anti-mouse CD370 antibody for Flow Cytometry (Clone: 10B4) please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD370 monoclonal antibodies:
Recombinant Anti-mouse CD370 monoclonal antibody (Clone: 10B4)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD370 bispecific antibodies:
Recombinant Anti-mouse CD370 / PD-L1 Bispecific Antibody (Clone: 10B4 / 10F.9G2.1)
Recombinant Anti-mouse CD370 / CD47 Bispecific Antibody (Clone: 10B4 / A4)
Syd Labs provides the following recombinant anti-mouse CD370 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD370 monoclonal antibody (Clone: 10B4) for flow cytometry
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