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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
The rat anti-mouse CD370 (Clec9A or DNGR-1) monoclonal antibody 10B4 (rat IgG2a kappa) reacts with the mouse CD370 protein. CLEC9A (C-type lectin domain family 9 member A), a transmembrane glycoprotein with a single extracellular C-type lectin domain (CTLD), plays a role in the immune response. As an activation receptor, CLEC9A is expressed on myeloid lineage cells, helps process material from dead cells, recognizes filamentous actin when cell membranes are damaged, and helps present antigens to cytotoxic T cells. CLEC9A has an intracellular cytoplasmic tail with potential signaling motifs. CLEC9A is expressed on a subset of BDCA3+ dendritic cells and CD16- monocytes in humans, and is expressed on CD8+ conventional DCs and plasmacytoid DCs in mice.
Our recombinant 10B4 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD370 monoclonal antibody (hybridoma clone name or number: 10B4).
The in vivo grade recombinant anti-mouse CD370 rat IgG2a monoclonal antibody was produced in mammalian cells.
Clone: 10B4.
Isotype: Rat IgG2a kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 10B4) specifically binds to mouse CD370.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD370 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD370 antibodies (clone of 10B4) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant rat IgG2a isotype control antibody
Anti-Dectin-1 antibody (clone 10B4) ameliorates experimental colitis in vivo
Heinsbroek, S. E., et al. Inflamm Bowel Dis. 2019 Mar 14;25(3):456-465. doi: 10.1093/ibd/izy305. PMID: 30213971
Mice were treated with 150 μg of anti-mouse CD370 antibody (clone 10B4, Bio X Cell) intravenously every 3 days starting on day 0 of DSS administration. Colitis was induced by 2.5% dextran sulfate sodium (DSS) in drinking water for 7 days. Colon length was measured as an indicator of disease severity. Anti-Dectin-1 treatment reduced inflammatory cell infiltration by 50%. Histological analysis showed improved mucosal integrity in treated mice.
Tags: anti-mouse CD370; activity of anti-mouse CD370 antibody
In vivo neutralization of Dectin-1 with 10B4 antibody enhances antitumor immunity in a mouse melanoma model
Chiba, S., et al. Cancer Res. 2017 Aug 1;77(15):4123-4134. doi: 10.1158/0008-5472.CAN-16-2794. PMID: 28687457
Anti-CD370 antibody (clone 10B4, Bio X Cell) was administered intraperitoneally at 100 μg per dose on days 3, 6, and 9 post-tumor implantation. B16F10 melanoma cells were injected subcutaneously into C57BL/6 mice. Tumor growth was monitored twice weekly using calipers. Dectin-1 blockade increased CD8+ T cell infiltration by 2-fold. Survival was prolonged by 20 days in the anti-CD370 group.
Tags: anti-mouse CD370 10B4 mAb; anti-mouse CD370 10B4 mAb in vivo
Role of Dectin-1 in host defense against Candida albicans assessed by in vivo blockade with clone 10B4
Marakalala, M. J., et al. Infect Immun. 2015 Feb;83(2):630-638. doi: 10.1128/IAI.01345-14. PMID: 25539822
Mice received 250 μg of anti-mouse CD370 antibody (clone 10B4, BioLegend) intraperitoneally on days -1 and 1 relative to Candida albicans infection. Systemic candidiasis was induced by intravenous injection of 1 × 10^5 CFU. Fungal burden was quantified in kidneys and spleen by CFU counts. Anti-Dectin-1 treatment increased fungal load by 40% in kidneys. Neutrophil recruitment was assessed by flow cytometry.
Tags: function of anti-mouse CD370 10B4; anti-mouse CD370 10B4 in cancer research
Dectin-1 deficiency or blockade with 10B4 antibody modulates allergic airway inflammation in mice
Ito, T., et al. J Allergy Clin Immunol. 2013 Apr;131(4):1138-46. doi: 10.1016/j.jaci.2013.01.023. PMID: 23436256
Anti-Dectin-1 antibody (clone 10B4, Bio X Cell) was injected intravenously at 200 μg per mouse on days 0, 2, and 4 of OVA challenge. Allergic airway inflammation was induced by OVA sensitization and challenge. Bronchoalveolar lavage fluid was collected for cell counts. Eosinophil infiltration was reduced by 60% in anti-Dectin-1-treated mice. Airway hyperresponsiveness was measured using plethysmography.
Tags: bioactivity of anti-mouse CD370 10B4; anti-mouse CD370 (10B4) in vivo antibody
Blockade of Dectin-1 signaling with monoclonal antibody 10B4 in experimental autoimmune arthritis
Yoshitomi, H., et al. Arthritis Rheum. 2009 Mar;60(3):737-46. doi: 10.1002/art.24371. PMID: 19273627
Anti-mouse CD370 antibody (clone 10B4, BioLegend) was administered intraperitoneally at 300 μg per week for 3 weeks. Collagen-induced arthritis was induced in DBA/1 mice by immunization with type II collagen. Joint swelling was scored weekly using a clinical arthritis index. Dectin-1 blockade reduced joint destruction by 50%. Serum cytokine levels were measured by ELISA.
Tags: anti-mouse CD370 antibody 10B4; clone 10B4 of anti-mouse CD370
For more references about anti-mouse CD370 antibody (Clone: 10B4), please contact our scientific support team with message@sydlabs.com.
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