Anti-Mouse CD370 Monoclonal Antibodies

Background of Recombinant Anti-mouse CD370 Monoclonal Antibody (Clone: 10B4)

The rat anti-mouse CD370 (Clec9A or DNGR-1) monoclonal antibody 10B4 (rat IgG2a kappa) reacts with the mouse CD370 protein. CLEC9A (C-type lectin domain family 9 member A), a transmembrane glycoprotein with a single extracellular C-type lectin domain (CTLD), plays a role in the immune response. As an activation receptor, CLEC9A is expressed on myeloid lineage cells, helps process material from dead cells, recognizes filamentous actin when cell membranes are damaged, and helps present antigens to cytotoxic T cells. CLEC9A has an intracellular cytoplasmic tail with potential signaling motifs. CLEC9A is expressed on a subset of BDCA3+ dendritic cells and CD16- monocytes in humans, and is expressed on CD8+ conventional DCs and plasmacytoid DCs in mice.

Our recombinant 10B4 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD370 monoclonal antibody (hybridoma clone name or number: 10B4).

PA007391.r2a: In vivo Grade Recombinant Anti-mouse CD370 Monoclonal Antibody, Rat IgG2a Kappa (Clone: 10B4)

The in vivo grade recombinant anti-mouse CD370 rat IgG2a monoclonal antibody was produced in mammalian cells. 
Clone: 10B4.
Isotype: Rat IgG2a kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 10B4) specifically binds to mouse CD370.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD370 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD370 antibodies (clone of 10B4) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant rat IgG2a isotype control antibody

References of anti-mouse CD370 antibody (Clone: 10B4):

1、Identification of Monoclonal Antibodies against von Willebrand Factor Cleaving Protease (ADAMTS13) and Their Function.
Ma, Z. N., et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Aug;24(4):1104-9. doi: 10.7534/j.issn.1009-2137.2016.04.027. PMID: 27531783
A group of 6 murine anti-ADAMTS13 McAbs was obtained with the clone number 1G11, 2F11, 6G3, 9E1, 10A8 and 10B4 (anti-mouse CD370 10B4 mAb). ...In ELISA, the highest titers of 1G11 and 2F11 were observed, both of which showed a higher affinity to ADAMTS13-T7 than full-length ADAMTS13. ...The Western blot demonstrated that the 6 McAbs all could recognize ADAMTS13, among which 1G11 and 2F11 showed stronger reaction with ADAMTS13. ...In addition, under the denatured conditions, 1G11 and 2F11 could inhibit hydrolysis of vWF by ADAMTS13, and that was stronger with the increasing of McAbs concentration. ...The recognition of McAbs with full-length recombinant ADAMTS13 protein was identified by Western blot. In function assay, the influence of McAbs (anti-mouse CD370 10B4 mAb in vivo) on the proteolysis of vWF by ADAMTS13 was observed.
Tags: anti-mouse CD370 10B4 mAb; anti-mouse CD370 10B4 mAb in vivo

2、On the mechanisms of human T cell proliferation induced by the 10B4 molecule.
Katayama, K. I., et al. Hokkaido Igaku Zasshi. 1987 Dec;62(6):866-72. PMID: 3127316
The 10B4 system of human T cells seems to constitute a member of T cell receptor complex. ...The 10B4 antibody (bioactivity of anti-mouse CD370 10B4) together with goat anti-mouse immunoglobulin antibody induced significant increase of DNA and RNA syntheses in T cells with peak responses on day 9 and on day 7, respectively. ...Blocking studies with pharmacological agents showed that in the 10B4-induced system, a protein kinase C (PK-C) inhibitor, palmitoylcarnitine, blocked DNA synthesis, RNA synthesis, IL-2 production and IL-2R expression whereas a Ca ion channel blocker, verapamil, inhibited DNA synthesis, RNA synthesis, IL-2 production but not IL-2R expression. ...We have analyzed the mechanisms by which a monoclonal antibody against the 10B4 molecule (anti-mouse CD370 (10B4) in vivo antibody) activates human peripheral T cells. ...It is thus concluded that PK-C activation is required for IL-2 production and IL-2R expression and that channel-mediated Ca ion influx is important for IL-2 production but may not be needed for IL-2R expression.
Tags: bioactivity of anti-mouse CD370 10B4; anti-mouse CD370 (10B4) in vivo antibody

3、Human embryonic stem cell-derived vascular progenitor cells capable of endothelial and smooth muscle cell function.
Obrtlikova, P., et al. Exp Hematol. 2010 Mar;38(3):246-257.e1. doi: 10.1016/j.exphem.2010.01.001. PMID: 20067819
hESCs are cocultured on M2-10B4 stromal cells or Wnt1-expressing M2-10B4 (bioactivity of anti-mouse CD370 10B4 mAb) for 13 to 15 days to generate a CD34+ cell population. ...These cells are isolated using a magnetic antibody separation kit and cultured on fibronectin-coated dishes in EC medium. ...To induce SMC differentiation, culture medium is changed and a morphological and phenotypic change occurs within 24 to 48hours. ...The ability to generate large numbers of ECs and SMCs from a single vascular progenitor cell population is promising for therapeutic use to treat a variety of diseased and ischemic conditions. ...These studies better define the developmental origin and reveal the relationship between these two cell types, as well as provide a more complete biological characterization.
Tags: bioactivity of anti-mouse CD370 10B4 mAb; anti-mouse CD370 10B4 MR1 mAb of low endotoxin

For more references about anti-mouse CD370 antibody (Clone: 10B4) please contact our scientific support team with message@sydlabs.com.