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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: NLDC-145.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD205 (DEC-205) monoclonal antibody (clone: NLDC-145) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: NLDC-145) specifically binds to mouse CD205 (DEC-205).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD205 (DEC-205) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD205 (DEC-205) monoclonal antibody of clone NLDC-145 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Targeting Antigens to Dec-205 on Dendritic Cells Induces Immune Protection in Experimental Colitis in Mice.
Wadwa, M., et al. Eur J Microbiol Immunol (Bp). 2016 Jan 27;6(1):1-8. doi: 10.1556/1886.2015.00048. PMID: 27141310
Among intestinal CD103+ DCs that are able to generate Foxp3+ Treg in the gut in a TGF-β/retinoic acid-dependent manner[8, 9], in recent years, the population of CD8α+ DEC-205+DCs has been identified as potent Treg inducer. ...DEC-205 is a c-type lectin receptor that is highly expressed on immature DCs and different epithelial cell types. ...It has been demonstrated extensively that targeting antigens to DEC-205 by specific antibody–antigen complexes leads to the efficient uptake and presentation of antigens on MHC I and MHC II complexes resulting in the activation of antigen-specific CD8+ and CD4+ T cells, respectively. ...Conjugation of the HA 110–120 peptide to the DEC-205-specific monoclonal antibody (mAb) was performed as previously described. ...In brief, α-DEC-205 (clone NLDC-145) or an IgG control antibody (clone GL117, kindly provided by Prof. Dr. Karsten Mahnke, University of Heidelberg) was purified from hybridoma supernatants and coupled to an activated HA 110–120 peptide using the heterobifunctional cross-linker SMCC (succinimidyl 4-( N-maleimidomethyl) cyclohexane-1-car-boxylate) (Pierce, Thermo Scientific, Bonn, Germany) according to the manufacturer’s protocol, resulting in the conjugates DEC-HA and GL117-HA, respectively.
Tags: anti-mouse CD205 (DEC-205) NLDC-145 antibody of low endotoxin; anti-mouse CD205 (DEC-205) (NLDC-145) in vivo antibody
For more references about Anti-mouse CD205 (DEC-205) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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