PA007595.r2a: In Vivo Grade Recombinant Anti-mouse CD205 (DEC-205) Monoclonal Antibody (Clone: NLDC-145), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: NLDC-145.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD205 (DEC-205) monoclonal antibody (clone: NLDC-145) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: NLDC-145) specifically binds to mouse CD205 (DEC-205).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD205 (DEC-205) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD205 (DEC-205) monoclonal antibody of clone NLDC-145 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD205 (DEC-205) antibody (Clone: NLDC-145):
Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
Trumpfheller C, et al. Methods Mol Biol. 2021;2225:99-110. doi: 10.1007/978-1-0716-1016-1_8. PMID: 33616089
The first step of the protocol is the purification of the antibody from the supernatant of the NLDC-145 hybridoma by affinity chromatography. In the past, we have successfully established cross-linking of the model antigen ovalbumin (OVA) and a DEC-205-specific IgG2a antibody (αDEC-205) for in vivo DC targeting studies in mice. The administration of low doses of the hybrid mAb together with DC maturation stimuli is able to activate specific T cells and induce production of high antibody titres for a number of different antigens. This protocol describes the chemical conjugation of a purified DEC-205-directed antibody with full-length protein antigens for targeting mouse dendritic cells in vitro and in vivo. The conjugation method is based on the formation of a thioether bond between the maleimide-activated protein and the thiolated antibody.
Tags: anti-mouse CD205 (DEC-205) NLDC-145; anti-mouse CD205 (DEC-205) NLDC-145 mAb
Improved cellular and humoral immune responses in vivo following targeting of HIV Gag to dendritic cells within human anti-human DEC205 monoclonal antibody
Chen L, et al. J Virol. 2010 Nov;84(21):10942-52. doi: 10.1128/JVI.00963-10. PMID: 20668230
Right: purified human IgG1 Fc alone (lane 1) or V5.hDEC205/hIgG1Fc fusion proteins (lane 2) produced by transfected CHO cells were analyzed on SDS-PAGE and stained by Coomassie blue. FACS labeling of CHO/hDEC205 and CHO/mDEC205 cells and human MoDCs stained at 1 μg/mL with human anti-hDEC205 mAbs, as well as the previously described NLDC145 rat anti-mDEC205 and MG38.2 mouse anti-hDEC205. The MoDCs were treated with/without LPS (100 ng/mL) to generate mature and immature DCs. (D) Lysates from CHO/Neo (lane 1), CHO/hDEC205 (lane 2), and CHO/mDEC205 (lane 3) cells were analyzed by Western blotting with mAbs 3G9 anti-hDEC205, NLDC145 anti-mDEC205, and anti-actin. To extend the concept to humans, we immunized human immunoglobulin-expressing mice with human DEC205 (hDEC205) extracellular domain.
Tags: anti-mouse CD205 (DEC-205) NLDC-145 in vivo; anti-mouse CD205 (DEC-205) NLDC-145 in animal model
CD205 antigen targeting combined with dendritic cell recruitment factors and antigen-linked CD40L activation primes and expands significant antigen-specific antibody and CD4(+) T cell responses following DNA vaccination of outbred animals
Khan S, et al. Immunology. 2012 Aug;136(4):519-33. doi: 10.1111/j.1365-2567.2012.03585.x. PMID: 22240344
Immunization of the calves with the CD205 antigen-targeting construct mixed with the cytokine constructs induced significant IFN-γ-secreting CD4(+) T-cells, CD4(+) T-cell proliferation, and antibody responses detectable within one week post-immunization. Comparative analysis of the immune responses observed one week post-priming versus the responses detected one week post-boost revealed that the average number of the IFN-γ-secreting CD4(+) T-cells observed in the calves immunized with the CD205 antigen targeting construct increased five-fold, the mean CD4(+) T-cell proliferation increased three-fold, whereas the mean IgG antibody titer increased two hundred-fold. The DNA construct was formulated with DNA-encoded Flt3L and GM-CSF for DC recruitment and the formulation was evaluated in MHC class II-matched calves. In a proof-of-concept study, we tested the immunogenicity of a single, low dose DNA vaccine targeting the CD205 receptor on dendritic cells in outbred calves. Targeting of antigens to the endocytic uptake receptor DEC205 resulted in enhanced antigen presentation by dendritic cells (DCs).
Tags: anti-mouse CD205 (DEC-205) NLDC-145 antibody in cancer research; anti-mouse CD205 (DEC-205) NLDC-145 in mouse tumor model
Broad T cell immunity to the LcrV virulence protein is induced by targeted delivery to DEC-205/CD205-positive mouse dendritic cells
Baumann G, et al. Vaccine. 2008 Jan 24;26(5):670-8. doi: 10.1016/j.vaccine.2007.11.020. PMID: 18081041
When compared to nontargeted standard protein vaccine, DC targeting greatly increased the efficiency for inducing IFN-γ-producing T cells. The targeted LcrV protein induced antibody responses to a similar extent as the F1-V subunit vaccine, but Th1-dependent IgG2a and IgG2c isotypes were observed only after anti-DEC-205:LcrV mAb immunization. Antibody response following αDEC-205:LcrV mAb immunization. (A) Anti-LcrV IgG antibody titers after 2 weeks of i.p. injection with graded doses of F1-V adsorbed in alum, αDEC-205:LcrV mAb, and LcrV protein in the presence of adjuvants (50 μg of poly IC and 25 μg of αCD40), or adjuvants alone. There is a need for a more efficient vaccine against the bacterium Yersinia pestis, the agent of pneumonic plague.
Tags: anti-mouse CD205 (DEC-205) NLDC-145 antibody of low endotoxin; anti-mouse CD205 (DEC-205) (NLDC-145) in vivo antibody
Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses
Deckers J, et al. J Immunol. 2012 Mar 1;188(5):2031-9. doi: 10.4049/jimmunol.1102955. PMID: 22291181
The injection site was immediately treated with imiquimod cream, or left untreated. At the indicated time points, targeting antibody was detected on permeabilised lymph nodes cells by anti-rat IgG / APC, and the different subset of DCs were distinguished by staining for CD11c, CD11b, CCR7, CD8α and Langerin. However, the relative contribution of these skin DC subsets to the induction of immune responses after Ag targeting has not been addressed in vivo. We show in this study that murine epidermal LCs and dermal DCs transport intradermally injected mAbs against the lectin receptor DEC-205/CD205 in vivo. The injection site was then topically treated with imiquimod cream or left untreated (n.t.).
Tags: anti-CD205 (DEC-205) clone NLDC-145; clone NLDC-145 of mouse CD205 (DEC-205) antibody
For more references about anti-mouse CD205 (DEC-205) antibody (Clone: NLDC-145), please contact our scientific support team with message@sydlabs.com.