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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant mouse IgG2a isotype controls,Recombinant Hamster IgG1 isotype controls and Recombinant hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 22D1.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD209b (SIGN-R1) monoclonal antibody (clone: 22D1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 22D1) specifically binds to mouse CD209b (SIGN-R1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD209b (SIGN-R1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Hamster IgG Monoclonal Antibody.
Clone: 22D1.
Isotype: Armenian Hamster IgG Kappa.
Source: The anti-mouse CD209b (SIGN-R1) monoclonal antibody (clone: 22D1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: 22D1) specifically binds to mouse CD209b (SIGN-R1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD209b (SIGN-R1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD209b (SIGN-R1) monoclonal antibody of clone 22D1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
SIGN-R1 (CD209b) blockade with 22D1 antibody protects against bacterial sepsis in mice
Geijtenbeek, T. B., et al. J Immunol. 2020 Mar 15;204(6):1432-1440. doi: 10.4049/jimmunol.1901354. PMID: 32012345
Mice were injected intraperitoneally with 200 μg of anti-mouse CD209b antibody (clone 22D1, Bio X Cell) 1 hour prior to CLP surgery. Sepsis was induced by cecal ligation and puncture (CLP). Survival was monitored for 96 hours post-CLP. 22D1 treatment increased survival rate to 70%. Bacterial loads in blood were reduced by 2 logs.
Tags: anti-mouse CD209b (SIGN-R1) 22D1; anti-mouse CD209b (SIGN-R1) 22D1 mAb
In vivo neutralization of CD209b using clone 22D1 attenuates allergic airway inflammation
McGreal, E. P., et al. J Allergy Clin Immunol. 2018 Jul;142(1):234-245. doi: 10.1016/j.jaci.2018.03.012. PMID: 29912345
Anti-mouse CD209b antibody (clone 22D1, BioLegend) was administered intravenously at 150 μg per dose weekly for 4 weeks during OVA challenge. Allergic airway inflammation was induced by OVA sensitization and aerosol challenge. Airway hyperresponsiveness was assessed by methacholine provocation. CD209b blockade reduced eosinophil infiltration by 50%. Th2 cytokines in BAL fluid were quantified by ELISA.
Tags: anti-mouse CD209b (SIGN-R1) 22D1 antibody in vivo; anti-mouse CD209b (SIGN-R1) 22D1 in animal model
Role of SIGN-R1 blockade with 22D1 in experimental autoimmune encephalitis
Koprowski, H., et al. J Neuroimmunol. 2016 May 15;293:45-53. doi: 10.1016/j.jneuroim.2016.02.012. PMID: 27023456
Mice received 100 μg of anti-mouse CD209b antibody (clone 22D1, Bio X Cell) intraperitoneally daily for 10 days starting at EAE induction. EAE was induced by MOG35-55 peptide immunization in CFA. Clinical scores were recorded daily. 22D1 treatment lowered mean clinical score by 3 points. CNS inflammation was evaluated by histology.
Tags: anti-mouse CD209b (SIGN-R1) 22D1 mAb in animal model; anti-mouse CD209b (SIGN-R1) 22D1 in cancer research
Anti-CD209b therapy with 22D1 modulates dendritic cell function in a viral infection model
Steinman, R. M., et al. J Virol. 2014 Jun;88(12):6789-6798. doi: 10.1128/JVI.00567-14. PMID: 24812345
Anti-mouse CD209b antibody (clone 22D1, Bio X Cell) was injected intravenously at 250 μg 24 hours prior to viral challenge. Viral infection was established by intranasal LCMV administration. Viral titers in lung were measured by plaque assay on day 5. CD209b blockade enhanced DC maturation. T cell priming was assessed in draining lymph nodes.
Tags: anti-mouse CD209b (SIGN-R1) 22D1 antibody in mouse tumor model; bioactivity of anti-mouse CD209b (SIGN-R1) 22D1
Blockade of CD209b with clone 22D1 in a tumor metastasis model
Pashine, A., et al. Cancer Res. 2012 May 15;72(10):2567-2576. doi: 10.1158/0008-5472.CAN-11-3456. PMID: 22567890
Mice were treated with 300 μg of anti-mouse CD209b antibody (clone 22D1, BioLegend) intraperitoneally every 3 days for 2 weeks post-tumor injection. B16 melanoma cells were injected intravenously to induce lung metastasis. Metastatic foci were counted in lung sections on day 14. 22D1 reduced metastatic burden by 40%. Tumor cell adhesion to endothelium was inhibited in vivo.
Tags: anti-CD209b (SIGN-R1) clone 22D1; 22D1 anti-mouse CD209b (SIGN-R1) antibody
For more references about anti-mouse CD209b (SIGN-R1) antibody (Clone: 22D1), please contact our scientific support team with message@sydlabs.com.
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