Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant mouse IgG2a isotype controls,Recombinant Hamster IgG1 isotype controls and Recombinant hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 22D1.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD209b (SIGN-R1) monoclonal antibody (clone: 22D1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 22D1) specifically binds to mouse CD209b (SIGN-R1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD209b (SIGN-R1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Hamster IgG Monoclonal Antibody.
Clone: 22D1.
Isotype: Armenian Hamster IgG Kappa.
Source: The anti-mouse CD209b (SIGN-R1) monoclonal antibody (clone: 22D1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: 22D1) specifically binds to mouse CD209b (SIGN-R1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD209b (SIGN-R1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD209b (SIGN-R1) monoclonal antibody of clone 22D1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
PD-1 promotes immune exhaustion by inducing antiviral T cell motility paralysis.
Zinselmeyer, B. H., et al. J Exp Med. 2013 Apr 8;210(4):757-74. doi: 10.1084/jem.20121416. PMID: 23530125
After the Fc block, cells were stained with cocktails of the following conjugated antibodies: CD279 biotin (RMP1-30), CD274 biotin (10F.962), F4/80 PerCP (BMB), CD45.2 APC (104), CD11c APC Cy7 (N418), CD45.1 Alexa Fluor 700 (A20), CD45.2 Alexa Fluor 700 (104; BioLegend), CD4 FITC (RM4-5), Thy1.1 PerCP (OX-7), CD8 PE (53–6.7), Thy1.2 PE (53–2.1; BD), CD11b PE Cy7 (M1/70), CD209b Alexa Fluor 647 (22D1; eBioscience), CD169 FITC (3D6.112; Serotec), CD8 Pacific Blue (5H10; Invitrogen), and gp38 PE (8.1.1; BioLegend). ...(A and B) The geometric mean fluorescent intensity (GMFI) of PD-L1 expression (mean ± SD) was quantified on splenic marginal zone macrophages (CD45+ Thy1.2− F4/80− CD209b+; MZM), myeloid dendritic cells (CD45+ Thy1.2− F4/80− CD169− CD209b− CD11c+ CD11b+; mDCs), red pulp macrophages (CD45+ Thy1.2− CD169− CD209b− CD11c− CD11b+ F4/80+; red pulp Mϕ), metallophilic macrophages (CD45+ Thy1.2− F4/80− CD169+; MMM), CD8α DCs (CD45+ Thy1.2− F4/80− CD169− CD209b− CD11b− CD11c+ CD8α+), CD8 T cells (CD45+ Thy1.2+ CD8α+), and CD4 T cells (CD45+ Thy1.2+ CD4+) at days 4 and 7 after Arm or CL13 infection. ...Spleens were homogenized and subsequently treated with TRIzol before performing a column-based RNA purification using the PureLink RNA mini kit (Invitrogen). ...The reactions were performed in triplicate, and RNA samples without reverse transcription were used as a negative control for each gene candidate. ...After blocking with 5% casein, ICAM-1-12-His labeled with Alexa Fluor 405 (AF405; Invitrogen; 200 molecules/µm2) was reacted with lipid bilayers in combination with H-2DbGP33-41-6-His (NIH tetramer core facility, Emory University; 185 molecules/um2).
Tags: anti-mouse CD209b (SIGN-R1) 22D1; anti-mouse CD209b (SIGN-R1) 22D1 mAb
The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
Kang, Y. S., et al. Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):215-20. doi: 10.1073/pnas.0307124101. PMID: 14694198
To establish a role for SIGN-R1 in this uptake, we selectively down-regulated expression of the lectin by pretreatment of the mice with SIGN-R1 antibodies, including a newly generated hamster monoclonal called 22D1. ...A soluble SIGN-R1 antigen of fusion between the extracellular portion of SIGN-R1 and mouse IgG Fc was produced, affinity purified from transfected mammalian cells, and used as antigen to generate a new hamster monoclonal antibody, 22D1, in the Hybridoma Core Facility at Mt. Sinai School of Medicine. ...After immunization of hamsters with SIGN-R1 fusion protein, we retrieved a stable monoclonal, named 22D1, that selectively bound to SIGN-R1 but not to cells transfected with other lectins (data not shown). ...The 22D1 antibody also immunoblotted SIGN-R1 (see below). However, the hamster antibody recognized a site distinct from the peptide recognized by rabbit PAb-C13 antibody. ...Similar results were obtained by FACS analysis, i.e., staining with 22D1 did not block staining with PAb-C13, and staining with PAb-C13 did not block reactivity with 22D1.
Tags: anti-mouse CD209b (SIGN-R1) 22D1 antibody in mouse tumor model; bioactivity of anti-mouse CD209b (SIGN-R1) 22D1
For more references about Anti-mouse CD209b (SIGN-R1) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf