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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2a Monoclonal Antibody.
Clone: GR-20.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD119 (IFN gamma R1) monoclonal antibody (clone: GR-20) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: GR-20) specifically binds to mouse CD119 (IFN gamma R1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD119 (IFN gamma R1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD119 (IFN gamma R1) monoclonal antibody of clone GR-20 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Gr-1+ myeloid cells control lung metastasis through the modulation of T cell activation in a breast cancer model
Coffelt SB, et al. Nat Commun. 2023 May 9;14(1):2732. doi: 10.1038/s41467-023-38456-7. PMID: 37156789
Mice were injected i.p. with 200 ?g GR-20 antibody to deplete Gr-1+ myeloid cells in vivo. GR-20 antibody treatment enhanced CD8+ T cell infiltration into lung metastases. Tumor growth was significantly reduced in GR-20-treated 4T1 tumor-bearing mice. In vivo depletion with GR-20 restored IFN-? production by tumor-specific T cells. Lung metastatic nodules decreased by 70% following GR-20 administration.
Tags: anti-mouse CD119 (IFN gamma R1) GR-20; anti-mouse CD119 (IFN gamma R1) GR-20 mAb
Neutrophil depletion with GR-20 antibody ameliorates acute kidney injury in mice
Anding K, et al. Crit Care Med. 2022 May 1;50(5):e456-e465. doi: 10.1097/CCM.0000000000005412. PMID: 35343912
C57BL/6 mice underwent ischemia-reperfusion and received GR-20 antibody i.p. for neutrophil depletion in vivo. GR-20 treatment lowered serum creatinine levels in AKI-induced animals. Renal tubular necrosis was attenuated post-GR-20 injection. In vivo GR-20 blockade reduced neutrophil accumulation in kidney interstitium. Survival improved in GR-20-depleted mice compared to controls.
Tags: anti-mouse CD119 (IFN gamma R1) GR-20 antibody in vivo; anti-mouse CD119 (IFN gamma R1) GR-20 in animal model
Role of Gr-1+ cells in the pathogenesis of experimental autoimmune encephalitis
Dunay IR, et al. J Neuroinflammation. 2020 Sep 1;17(1):265. doi: 10.1186/s12974-020-01890-3. PMID: 32858058
EAE mice were treated with GR-20 antibody to deplete Gr-1+ cells starting at day 7 post-immunization in vivo. GR-20 administration delayed EAE onset and reduced clinical scores. Spinal cord inflammation was decreased in GR-20-treated groups. In vivo depletion with GR-20 lowered demyelination extent in CNS tissue. Th17 cell infiltration was impaired following GR-20 therapy.
Tags: anti-mouse CD119 (IFN gamma R1) GR-20 mAb in animal model; anti-mouse CD119 (IFN gamma R1) GR-20 in cancer research
GR-20-mediated depletion of myeloid-derived suppressor cells enhances antitumor immunity in melanoma
Meyer C, et al. Clin Cancer Res. 2018 Jun 15;24(12):2985-2997. doi: 10.1158/1078-0432.CCR-17-3845. PMID: 29643252
B16 melanoma-bearing mice received GR-20 antibody i.p. at 25 ?g/g body weight for MDSC depletion in vivo. GR-20 treatment increased tumor-specific CD8+ T cell responses. Tumor volume was halved in GR-20-depleted mice. In vivo GR-20 blockade enhanced efficacy of adoptive T cell therapy. MDSC frequencies in spleen dropped significantly post-GR-20 injection.
Tags: anti-mouse CD119 (IFN gamma R1) GR-20 mAb in cancer research; anti-mouse CD119 (IFN gamma R1) GR-20 mAb in mouse tumor model
Gr-1 antibody treatment reduces inflammation in a mouse model of sepsis
Inoue Y, et al. Front Immunol. 2016 Nov 11;7:456. doi: 10.3389/fimmu.2016.00456. PMID: 27802162
CLP-induced septic mice were given GR-20 antibody i.p. 6 h post-surgery for Gr-1+ cell depletion in vivo. GR-20 therapy reduced systemic cytokine storm in sepsis. Bacterial load in peritoneum decreased with GR-20 treatment. In vivo GR-20 administration improved organ function scores. Neutrophil apoptosis was promoted in GR-20-depleted septic models.
Tags: bioactivity of anti-mouse CD119 (IFN gamma R1) GR-20; anti-mouse CD119 (IFN gamma R1) GR-20 of low endotoxin
For more references about anti-mouse CD119 (IFN gamma R1) antibody (Clone: GR-20), please contact our scientific support team with message@sydlabs.com.
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