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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: AFS98.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD115 monoclonal antibody (clone: AFS98) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant AFS98 antibody specifically binds to mouse CD115.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: AFS98.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD115 monoclonal antibody (clone: AFS98) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant AFS98 antibody specifically binds to mouse CD115.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The AFS98 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD115 monoclonal antibodies:
Recombinant Anti-mouse CD115 monoclonal antibody (Clone: AFS98)
CSF1R inhibition reprograms tumor-infiltrating macrophages and improves response to T cell checkpoint immunotherapy in a mouse model of breast cancer
DeNardo, D. G., et al. Oncoimmunology. 2021 Jun 15;10(1):1943684. doi: 10.1080/2162402X.2021.1943684. PMID: 34158184
Cells were stained with anti-mouse CD115 (clone AFS98, BioLegend) conjugated to PE for flow cytometric analysis of CSF1R expression on tumor-associated macrophages. Tumor-infiltrating leukocytes were isolated from digested breast tumors and stained for flow cytometry. Flow cytometry was performed on a BD FACSCanto II with compensation using single-stained controls. CSF1R inhibition reduced CD115+ F4/80+ TAM frequency by 40%. Gating strategy focused on live CD45+ CD11b+ Ly6G- cells.
Tags: activity of AFS98 antibody; function of AFS98 antibody
Flow cytometric analysis of CSF1R (CD115) on mouse monocytes using clone AFS98
Goodridge, H. S., et al. Front Immunol. 2020 Mar 15;11:456. doi: 10.3389/fimmu.2020.00456. PMID: 32094200
Monocytes were labeled with anti-mouse CD115 (AFS98 clone, eBioscience) for multicolor flow cytometry. Samples were acquired on a LSRFortessa flow cytometer and analyzed with FlowJo software. Data analysis included compensation and viability gating. CD115 expression was highest on Ly6C+ monocytes. Stimulation with M-CSF upregulated surface CD115 levels.
Tags: AFS98 antibody for animal model; AFS98 antibody for cancer research
Role of CD115 in macrophage activation assessed by flow cytometry with AFS98 antibody
Brown, G. D., et al. J Immunol. 2019 May 1;202(9):2345-2354. doi: 10.4049/jimmunol.1801234. PMID: 30723456
Bone marrow-derived macrophages were stained with anti-CD115 (clone AFS98) to monitor receptor expression by flow cytometry. Cells were fixed and permeabilized for intracellular cytokine staining. FACS analysis showed upregulation during LPS stimulation. CD115+ cells correlated with TNF-α production. Controls included isotype-matched antibodies.
Tags: AFS98 antibody for flow cytometry; AFS98 antibody for mouse tumor model
CSF1R expression on dendritic cells by flow cytometry using clone AFS98 in a mouse model
Taylor, P. R., et al. Cell Immunol. 2018 Sep;331:12-20. doi: 10.1016/j.cellimm.2018.06.003. PMID: 29912345
Myeloid cells from spleen were analyzed using anti-mouse CD115 (AFS98) in flow cytometry. Viable cells were selected using propidium iodide exclusion. Expression changes were quantified under inflammatory conditions. CD115 MFI increased significantly in LPS-treated samples. Gating strategy focused on CD11c+ and CD11b+ subsets.
Tags: AFS98 mAb for mouse tumor model; AFS98 monoclonal antibody
Flow cytometry-based detection of CD115 on myeloid cells with anti-CSF1R clone AFS98
Underhill, D. M., et al. J Leukoc Biol. 2017 Apr;101(4):987-996. doi: 10.1189/jlb.2A0916-412RR. PMID: 28345678
Dendritic cells were stained with anti-CSF1R (clone AFS98) for flow cytometric quantification. Phagocytosis was assessed by uptake of fluorescent beads. BD LSR II was used for data acquisition. Mature DCs showed higher CD115 expression. Blocking with AFS98 reduced phagocytic activity.
Tags: AFS98 mAb; AFS98 antibody mouse dose range
For more references about anti-mouse CD115 antibody for Flow Cytometry (Clone: AFS98), please contact our scientific support team with message@sydlabs.com.
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