Anti-Mouse CD115 Monoclonal Antibodies

Background of Anti-mouse CD115 Monoclonal Antibody

The rat anti-mouse CD115 (CSF1R or M-CSFR) monoclonal antibody AFS98 (rat IgG2a kappa) reacts with the mouse CD115 protein. CSF1R is found in the outer membrane of certain cell types, including microglia and macrophages. CSF1R controls the development, survival, and maintenance of microglia, and is involved in the proliferation, differentiation, and activation of macrophages.

Our recombinant AFS98 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD115 monoclonal antibody (hybridoma clone name or number: AFS98).

PA007394.r2a: In vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98)

The in vivo grade recombinant anti-mouse CD115 rat IgG2a monoclonal antibody was produced in mammalian cells. 
Clone: AFS98.
Isotype: Rat IgG2a kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: AFS98) specifically binds to mouse CD115.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD115 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD115 antibodies (clone of AFS98) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD115 Monoclonal Antibody:

Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection.
Kaminsky, L. W., et al. J Virol. 2015 Oct;89(19):9974-85. doi: 10.1128/JVI.01024-15. PMID: 26202250
For depletion of CD115+ cells, mice were injected i.p. every other day starting at day −5 with 3 mg AFS98 antibody (anti-CD115 depleting antibody) in 200 μl 0.1% BSA- HBSS. ...We administered the anti-CD115 (CSF-1R)-blocking antibody AFS98, a monoclonal antibody commonly used to target tissue-resident cells of the monocyte-macrophage lineage, with high availability throughout the body. ...As expected, macrophages were partially, but not fully, depleted in the spleens of AFS98-injected mice, as not all macrophage populations within secondary lymphoid organs are sensitive to anti-CD115 treatment. ...Neither mice treated with anti-CD115 nor mice treated with vehicle control died upon ECTV challenge (Fig. 1G), indicating that tissue-resident macrophage populations were not required for anti-ECTV immunity. ...(F and G) WT mice were injected i.p. with 3 mg/mouse anti-CD115 depleting antibody or vehicle control every other day starting at day −5 and infected with ECTV.
Tags: anti-mouse CD115 AFS98; anti-mouse CD115 AFS98 in vivo

CD11c(+) monocyte/macrophages promote chronic Helicobacter hepaticus-induced intestinal inflammation through the production of IL-23.
Arnold, I. C., et al. Mucosal Immunol. 2016 Mar;9(2):352-63. doi: 10.1038/mi.2015.65. PMID: 26242598
Anti-CSF-1R mAb was purified from the culture supernatants of AFS98 hybridoma, kindly provided by Dr Miriam Merad (Mount Sinai School of Medicine, New York, NY). ...Mice were injected intraperitoneally with anti-CSF-1R mAb (AFS98) or rat IgG1 isotype control (HRPN, BioXcell, West Lebanon, NH) at doses of 1 mg per mouse on day −4 followed by 0.3 mg per mouse from day −3 to day 0. ...We thank Paresh Thakker for providing Il23afl/fl mice, Miriam Merad for providing the AFS98 hybridoma cell line, Fanny Franchini for technical assistance, Philip Ahern for his comments and suggestions regarding the manuscript, Helen Ferry for flow cytometry cell sorting, and Richard Stillion for histology. I.C.A. was supported by a Swiss National Science Foundation fellowship. S.M. ...DC-derived IL-23 has been shown to be involved in both homeostatic and inflammatory mucosal functions. ...Overall, our observation that intestinal CX3CR1-expressing CD11c+ cells are the major source of IL-23 in a physiological model of bacteria-driven colitis confirms the pathogenic potential of these cells during colitis and uncovers a novel mechanism through which they might perpetuate chronic inflammation.
Tags: anti-mouse CD115 AFS98 antibody in vivo; anti-mouse CD115 AFS98 in animal model

IL-3 and CSF-1 interact to promote generation of CD11c+ IL-10-producing macrophages.
Sheng, K.-C., et al. PLoS One. 2014 Apr 17;9(4):e95208. doi: 10.1371/journal.pone.0095208. PMID: 24743235
In some experiments, cells were co-incubated with 5 µg/ml CSF-1R blocking antibody anti-CD115 (AFS98) (eBioscience) [26] or isotype control antibody (Rat IgG2a κ) (eBioscience). ...We further investigated the mechanism by which CSF-1 modulates the IL-3-mediated hematopoietic pathway. CD11c+ cell generation mediated by IL-3 was significantly inhibited when CSF-1R was blocked with AFS98. ...In contrast, the AFS98 antibody had no effect on the generation of CD11c+ cells induced by GM-CSF. ...In a positive control, AFS98 blocked the action of CSF-1, resulting in a similar level of CD11c+ cell generation to that seen in the AFS98 treated IL-3 culture. ...BM cells were isolated and cultured with GM-CSF in the presence of AFS98 or the isotype control antibody in triplicates in a 24 well plate.
Tags: anti-mouse CD115 AFS98 in cancer research; anti-mouse CD115 AFS98 in mouse tumor model

Coordinate regulation of tissue macrophage and dendritic cell population dynamics by CSF-1.
Tagliani, E., et al. J Exp Med. 2011 Aug 29;208(9):1901-16. doi: 10.1084/jem.20110866. PMID: 21825019
CSF-1 is produced by a variety of stromal and epithelial cell types and signals through the CSF-1R (CD115) tyrosine kinase receptor (encoded by the Csf1r/c-fms proto-oncogene) expressed by cells of the mononuclear phagocyte lineage. ...Although no absolute deficits in these two latter subsets have been noted in the absence of CSF-1 signaling, both CD11bhi DCs and pre-DCs express CD115, and Csfr1−/− mice show a relative loss of peripheral CD11bhi CD103− DCs as compared with CD11blo CD103+ DCs. ...Bone marrow mononuclear cells from CX3CR1GFP CD45.1 mice were enriched by granulocyte and erythrocyte depletion on a Ficoll density gradient as described previously (Varol et al., 2009a), and 3 × 106 sorted Ly6Chi Mos (GFP+ CD11b+ Ly6Chi CD115hi cells) were i.v. injected into unconditioned pregnant recipient mice. ...Author contributions: E. Tagliani and C.-S. Tay performed experiments, P. Nancy provided key reagents, and C. Shi and E.G. ...We have studied Mϕ and DC population dynamics in the mouse uterus during the first half of gestation, when the implanted uterus grows ∼15-fold in 8 d. Most clearly, our results provide a framework for understanding how local Mϕ tissue densities are specified by in situ levels of CSF-1 activity.
Tags: function of anti-mouse CD115 AFS98 antibody; bioactivity of anti-mouse CD115 AFS98

Antibody blockade of c-fms suppresses the progression of inflammation and injury in early diabetic nephropathy in obese db/db mice.
Lim, A. K. H., et al. Diabetologia. 2009 Aug;52(8):1669-79. doi: 10.1007/s00125-009-1399-3. PMID: 19466391
Obese, type 2 diabetic db/db BL/KS mice with established albuminuria were treated with a neutralising anti-c-fms monoclonal antibody (AFS98) or isotype matched control IgG from 12 to 18 weeks of age and examined for renal injury. ...Treatment with AFS98 did not affect obesity, hyperglycaemia, circulating monocyte levels or established albuminuria in db/db mice. ...However, AFS98 did prevent glomerular hyperfiltration and suppressed variables of inflammation in the diabetic kidney, including kidney macrophages (accumulation, activation and proliferation), chemokine CC motif ligand 2 levels (mRNA and urine protein), kidney activation of proinflammatory pathways (c-Jun amino-terminal kinase and activating transcription factor 2) and Tnf-α (also known as Tnf) mRNA levels. ...In addition, AFS98 decreased the tissue damage caused by macrophages including tubular injury (apoptosis and hypertrophy), interstitial damage (cell proliferation and myofibroblast accrual) and renal fibrosis (Tgf-β1 [also known as Tgfb1] and Col4a1 mRNA). ...AFS98 is a rat anti-mouse c-fms mAb (IgG2a) which neutralises the activity of c-fms by preventing the binding of CSF-1.
Tags: anti-mouse CD115 antibody (AFS98); anti-CD115 clone AFS98

For more references about Anti-mouse CD115 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.