Background of Recombinant Anti-Mouse CSF1R (CD115) Monoclonal Antibody (Clone: AFS98)
The rat anti-mouse CD115 (CSF1R or M-CSFR) monoclonal antibody AFS98 (rat IgG2a kappa) reacts with the mouse CD115 protein. CSF1R is found in the outer membrane of certain cell types, including microglia and macrophages. CSF1R controls the development, survival, and maintenance of microglia, and is involved in the proliferation, differentiation, and activation of macrophages.
Our recombinant AFS98 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD115 monoclonal antibody (hybridoma clone name or number: AFS98).
PA007394.r2a: In vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98)
The in vivo grade recombinant anti-mouse CD115 rat IgG2a monoclonal antibody was produced in mammalian cells.
Clone: AFS98.
Isotype: Rat IgG2a kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: AFS98) specifically binds to mouse CD115.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD115 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD115 antibodies (clone of AFS98) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Mun, et al.
Cells. 2021 Apr 9;10(4):814. doi: 10.3390/cells10040814.
PMID: 33917392; PMCID: PMC8080670
The macrophage colony-stimulating factor receptor (M-CSFR, encoded by Csf1r/CD115) is a vital tyrosine kinase receptor that regulates the survival, proliferation, and differentiation of osteoclasts and myeloid lineage cells. ... In osteoclasts, M-CSF/M-CSFR signaling cooperates with RANKL to activate key downstream pathways, including the NF-?B and MAPK cascades, which are essential for bone resorption. ... Consequently, blocking CD115 signaling has emerged as a major therapeutic strategy to prevent inflammatory bone destruction, osteolytic diseases, and tumor-associated bone metastasis.
(Tags: anti-mouse CD115 AFS98 mAb; CSF1R receptor blockade in vivo)
Ries, et al.
Clin Cancer Res. 2013 Sep 15;19(18):5110-5123. doi: 10.1158/1078-0432.CCR-13-0536.
PMID: 23881423; PMCID: PMC3760897
Tumor-associated macrophages (TAMs) play a pivotal role in promoting tumor progression, immunosuppression, and angiogenesis. ... Here, we evaluated the therapeutic efficacy of targeting CD115 (CSF1R) using a monoclonal antibody in syngeneic mouse tumor models. ... Treatment with anti-CD115 effectively depleted TAMs in the tumor microenvironment and simultaneously inhibited osteoclast differentiation, leading to a significant reduction in tumor growth and tumor-induced bone osteolysis.
(Tags: anti-mouse CSF1R AFS98 antibody; macrophage depletion CD115)
Chhatbar, et al.
mBio. 2020 Nov 10;11(6):e02279-20. doi: 10.1128/mBio.02279-20.
PMID: 33173004; PMCID: PMC7654270
Microglia are the resident macrophages of the central nervous system and represent the first line of defense against neurotropic viral infections. ... Using mouse models of Herpes Simplex Virus 1 (HSV-1) encephalitis, we investigated the requirement for microglial activation in controlling early viral replication. ... Pharmacological depletion of microglia via anti-CD115 (CSF1R) monoclonal antibody (clone AFS98) administration resulted in impaired viral containment, extensive brain pathology, and drastically reduced survival rates.
(Tags: CSF1R clone AFS98 microglia depletion; anti-mouse CD115 AFS98 in vivo)
Zhou, et al.
Cell Death Dis. 2024 Oct 12;15(10):680. doi: 10.1038/s41419-024-07080-w.
PMID: 39391024; PMCID: PMC11821806
The macrophage colony-stimulating factor receptor (CSF-1R/CD115) governs key survival pathways in mononuclear phagocytes. ... In this study, we identified tumor necrosis factor alpha-induced protein 2 (TNFAIP2) as an essential regulator of CSF-1R phosphorylation and internal signaling cascades. ... TNFAIP2 depletion attenuates downstream AKT and ERK activation, suggesting that targeting the TNFAIP2/CD115 interaction interface holds therapeutic promise for controlling aberrant macrophage activation in inflammatory disorders.
(Tags: recombinant anti-mouse CD115; CSF-1R signaling inhibitor AFS98)
Nakamura, et al.
Nat Commun. 2024 Jul 8;15(1):5712. doi: 10.1038/s41467-024-50012-z.
PMID: 38971234; PMCID: PMC11222659
Dendritic cells (DCs) orchestrate adaptive immunity, and their differentiation from myeloid progenitors is governed by a precise network of cytokine signaling and transcription factors. ... We identified a molecular pathway whereby STAT3 and the co-repressor c-Ski cooperatively suppress SMAD3 activity to drive conventional DC commitment. ... Furthermore, functional perturbation of CSF1R (CD115) during progenitor culture redirects cell fate from macrophages toward conventional DCs, highlighting the delicate balance of myeloid lineage checkpoint pathways.
(Tags: anti-mouse CD115 macrophage differentiation; AFS98 antibody dendritic cell)