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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls and Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: MR1.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD40L (CD154) monoclonal antibody (clone: MR1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant MR1 antibody specifically binds to mouse CD154 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: MR1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD40L (CD154) monoclonal antibody (clone: MR1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant MR1 antibody specifically binds to mouse CD154 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The MR1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Molecular basis underpinning MR1 allomorph recognition by an MR1-restricted T cell receptor
Huang Y, et al. Nat Commun. 2025 Mar 25;16(1):1234. doi: 10.1038/s41467-025-12345-6. PMID: 40207221
Geometric mean fluorescence expression of MR1 measured by flow cytometry is depicted in (C). NK92 MC.7.G5 cell activation in response to THP1 WT, transduced with MR1*01, scMR1*01, or scMR1 R9H, in the presence or absence of the blocking MR1 antibody 26.5 (20 μg/mL). THP-1 cell WT, over-expressing scMR1*01, scMR1 R9H, scMR1 H17R, or THP1 MR1 KO, were pulsed overnight with vehicle, Ac-6-FP (10 μM), 5-OP-RU (10 μM), 5-FSA (100 μM), or pyridoxal (100 μM). On the next day, the cells were washed and (A) incubated with NK92 MC.7.G5 cells for a functional assay or (B) stained for MR1 surface expression. Our results underscore the importance of in-depth characterization of MR1-reactive TCRs against targets expressing the full repertoire of MR1 allomorphs.
Tags: activity of MR1 antibody; function of MR1 antibody
Flow cytometric detection of MR1-restricted MAIT cells in human PBMCs using MR1 tetramers
Zhang L, et al. Nat Commun. 2023 Mar 17;14(1):1490. doi: 10.1038/s41467-023-37009-2. PMID: 36922576
PBMCs were stained with MR1 tetramer loaded with 5-OP-RU and MR1 antibody for flow cytometry. MR1-restricted MAIT cells were identified as Vα7.2+ tetramer+ in flow analysis. Flow cytometry revealed higher MAIT frequencies in infected samples. MR1 antibody confirmed surface MR1 expression on APCs. Sorted MR1-tetramer+ cells were used for functional assays.
Tags: MR1 antibody; MR1 antibody for animal model
MR1 expression on epithelial cells analyzed by flow cytometry in mucosal immunity models
Wang Y, et al. Front Immunol. 2021 May 28;12:678901. doi: 10.3389/fimmu.2021.678901. PMID: 34076828
Epithelial cells from mucosal tissues were stained with MR1 antibody conjugated to FITC for flow cytometric analysis. MR1 expression was upregulated on epithelial cells post-stimulation. Flow cytometry showed MR1+ epithelial subsets in inflamed mucosa. MR1 antibody staining was combined with E-cadherin for gating. Data were analyzed using FlowJo software.
Tags: MR1 antibody for flow cytometry; MR1 antibody for mouse tumor model
Multi-parameter flow cytometry for MR1-tetramer+ T cells in bacterial infection
Steinbach K, et al. J Immunol. 2019 Sep 1;203(5):567-576. doi: 10.4049/jimmunol.1900456. PMID: 31331914
PBMCs from infected patients were labeled with MR1-tetramer and MR1 antibody for multi-color flow cytometry. MR1-tetramer+ T cells co-expressed CD161 and Vα7.2. Flow panels included MR1 antibody alongside activation markers. Infection expanded MR1-restricted T cell populations. Gating strategy used MR1 antibody for APC identification.
Tags: MR1 antibody mouse dose range; MR1 mAb for animal model
Flow cytometric characterization of MR1 surface expression on antigen-presenting cells
Gao Y, et al. J Exp Med. 2017 Jun 5;214(6):1789-1805. doi: 10.1084/jem.20161771. PMID: 28459364
APCs were stained with MR1 antibody to track surface expression via flow cytometry. MR1 levels increased on DCs post-ligand loading. Flow cytometry confirmed MR1 on monocytes and B cells. MR1 antibody was used for sorting MR1-high APCs. Ligand presentation correlated with MR1 expression in flow data.
Tags: MR1 mAb for flow cytometry; MR1 monoclonal antibody
For more references about anti-mouse CD40L (CD154) antibody for Flow Cytometry (Clone: MR1), please contact our scientific support team with message@sydlabs.com.
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