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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: M/K-2.7.
Isotype: Rat IgG1 kappa.
Source: The anti-mouse CD106 (VCAM-1) monoclonal antibody (clone: M/K-2.7) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M/K-2.7) specifically binds to mouse CD106 (VCAM-1) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD106 (VCAM-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD106 (VCAM-1) monoclonal antibody of clone M/K-2.7 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Artery-Associated Sympathetic Innervation Drives Rhythmic Vascular Inflammation of Arteries and Veins.
de Juan, A., et al. Circulation. 2019 Sep 24;140(13):1100-1114. doi: 10.1161/CIRCULATIONAHA.119.040232. PMID: 31401849
Animals were injected intravenously with 200 ?g of the following blocking antibodies (BioxCell): anti-CD54 (ICAM-1; clone YN1/1.7.4), anti-CD106 (VCAM-1; clone M/K-2.7), or their isotypes (for ICAM-1 clone LTF-2 and for VCAM-1 clone HRPN) or antagonists against the chemokine receptors CXCR2 and CCR2 (5 mg/kg, Tocris) 1 hour before stimulation with TNF-? and 3 hours before in vivo imaging experiments. ...Mice were anesthetized with fentanyl (0.5 mg/kg), midazolam (5 mg/kg), and medetomidine (0.5 mg/kg) via intraperitoneal injection, and a catheter was inserted into the femoral artery for the administration of FITC-coupled dextran (2.5% 500 kDa). ...We set out to perform a detailed investigation into time-of-day?dependent leukocyte recruitment patterns to arteries and veins in an acute inflammatory scenario. ...Using multichannel fluorescence intravital microscopy of the cremaster muscle microcirculation, we detected a similar time-dependent leukocyte adhesion pattern in smaller arterioles and venules. ...These data provide for the first time evidence that inflammatory leukocyte recruitment to arteries and veins is diurnally regulated with distinct rhythmicity depending on the type of vascular bed.
Tags: anti-mouse CD106 (VCAM-1) MK-2.7; anti-mouse CD106 (VCAM-1) MK-2.7 antibody
Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury.
Kapitsinou, P. P., et al. J Clin Invest. 2014 Jun;124(6):2396-409. doi: 10.1172/JCI69073. PMID: 24789906
Because of its highly specialized vascular anatomy and the relatively low tissue pO2 levels, the kidney is particularly susceptible to hypoxic injury, which in hospitalized patients frequently results in acute ischemic renal failure, a condition associated with high mortality and transition to chronic kidney disease (CKD). ...Like other cell types, ECs respond to changes in tissue pO2 levels by multiple hypoxic signaling mechanisms. ...In the noninjured kidney, HIF-1? has been detected in tubular epithelium and in ECs following exposure to acute hypoxia, while HIF-2? is predominantly expressed in ECs and glomerular cells as well as in peritubular interstitial cells, where it regulates erythropoietin (EPO) synthesis. ...Furthermore, measurements of renal function parameters, such as blood urea nitrogen (BUN), serum electrolytes, urine albumin, and Epo levels, revealed no significant differences between Hif1aHif2a?/? and Cre-negative Hif1aflox/floxHif2aflox/flox mice, herein referred to as Cre? littermate controls (Supplemental Table 1). ...Because ECs regulate inflammation in injured tissues through modulation of neutrophil adhesion, we next examined mRNA expression levels of endothelial adhesion molecules by real-time PCR.
Tags: anti-mouse CD106 (VCAM-1) MK-2.7 in vivo; anti-mouse CD106 (VCAM-1) MK-2.7 antibody in vivo
PLZF induces an intravascular surveillance program mediated by long-lived LFA-1-ICAM-1 interactions.
Thomas, S. Y., et al. J Exp Med. 2011 Jun 6;208(6):1179-88. doi: 10.1084/jem.20102630. PMID: 21624939
Blocking antibodies against mouse CD11a (LFA-1; M17/4), CD54 (ICAM-1; YNI.7.4), CD49d (VLA-4; PS/2), and CD106 (VCAM-1; M/K-2.7) and rat IgG2a Control (2A3) were purchased from BioXCell and injected iv 3 h before tissue harvest. ...Using parabiotic mice, we demonstrated that, despite their intravascular location, most liver NKT cells failed to recirculate. ...This unprecedented lifelong intravascular residence could be induced in conventional CD4 T cells by the sole expression of promyelocytic leukemia zinc finger (PLZF), a transcription factor specifically expressed in the NKT lineage. ...A majority of NKT cells recognize the same microbial lipids characterized by a galactose in ? linkage to a ceramide or a diacylglycerol backbone. ...NKT cells are not only involved in microbial defense, as expected, but they also contribute to immune responses during viral infections, cancer, and various inflammatory and allergic states.
Tags: anti-mouse CD106 (VCAM-1) MK-2.7 antibody in cancer research; anti-mouse CD106 (VCAM-1) MK-2.7 in mouse tumor model
For more references about Anti-mouse CD106 (VCAM-1) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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