PA007711.m1: In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa
Recombinant mouse IgG1 monoclonal antibody.
Clone: 1B2.
Isotype: Mouse IgG1 kappa.
Source: The anti-mouse 2C TCR monoclonal antibody (clone: 1B2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 1B2) specifically binds to mouse 2C TCR .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse 2C TCR protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse 2C TCR monoclonal antibody of clone 1B2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Woo, S. R., et al.
Immunity. 2014 Nov 20;41(5):830-42.
PMID: 25517615
Because MyD88 can function in a T cell-intrinsic fashion, we performed adoptive transfer of wild-type (WT) CFSE-labeled 2C TCR Tg T cells (specific for the model antigen SIY) into WT or Myd88-/- mice and challenged with B16.SIY tumors. To evaluate the mechanism by which the absence of host STING resulted in impaired antitumor T cell responses, we adoptively transferred CFSE-labeled 2C TCR Tg T cells into WT and STING-deficient mice and measured T cell proliferation. Splenocytes from recipient mice were analyzed after staining with anti-mouse CD8α-APC and biotin-labeled anti-2C-TCR (1B2) with SA-PE by flow cytometry to assess CFSE dilution.
(Tags: activity of Anti-Mouse 2C TCR Monoclonal; Anti-Mouse 2C TCR Monoclonal)
Ophir, E., et al.
Blood. 2013 Feb 14;121(7):1220-8.
PMID: 23223359
Stained 2C CD8+ HTCs and CB6 Tcms (recognized specifically by the 2C TCR transgene) were adoptively transferred into lethally and sublethally irradiated C57BL/6 mice. The number of 2C TCR Tg cells used is within the physiologic range observed for naive antigen-specific precursors. In addition, the 2C-derived CD8+ cells can be identified using the clonotypic 1B2 antibody directed against their transgenic TCR.
(Tags: Anti-Mouse 2C TCR Monoclonal antibody for animal model; Anti-Mouse 2C TCR Monoclonal antibody for cancer research)
Lu, D., et al.
Nat Commun. 2023 Oct 12;14(1):6389.
PMID: 37828002
1-2C TCR-T cells show tumor suppression efficacy in a tumor-bearing mouse model and improved inhibition of tumor growth in combination with anti-PD-1 antibodies. Of note, 1-2C TCR is a public TCR that presents in each of the four mice and dominated KRAS-G12V-9 specific TCRs. The 1-2C TCR has higher binding affinity and immune responsiveness against tumor cells than the 3-2E TCR, and 1-2C was thus selected for further in vivo anti-tumor evaluations.
(Tags: Anti-Mouse 2C TCR Monoclonal antibody for mouse tumor model; Anti-Mouse 2C TCR Monoclonal for animal model)
Nishimura, H., et al.
Immunity. 1999 Aug;11(2):141-51.
PMID: 10485649
Anti-2C TCRαβ clonotypic mAb, 1B2, and anti-mouse PD-1 mAb (J43) were biotinylated. For immunochemistry, cryosections were pretreated with 2% BSA in PBS/0.05% Tween and incubated with biotinylated CD8α or 1B2 overnight. Unlike most of the lupus-prone mice, common autoantibodies represented by anti-double strand DNA (dsDNA) and rheumatoid factor (RF) were hardly detected in B6-PD-1-/- mice.
(Tags: Anti-Mouse 2C TCR Monoclonal for flow cytometry; Anti-Mouse 2C TCR Monoclonal in vivo)
Zoete, V., et al.
Front Immunol. 2013 Sep 12;4:268.
PMID: 24062738
Using the well-characterized 2C TCR/SIYR/H-2Kb structure as a model system, we demonstrated that a binding free energy decomposition based on the MM-GBSA approach provides a detailed and reliable description of the TCR/pMHC interactions. To assess the ability of the MM-GBSA approach to identify quantitatively the hot-spot residues for the TCR/pMHC association, we performed a study of the 2C TCR/SIYR/H-2Kb system using both the CAS and BFED methods. Our results also showed that BFED provided a more detailed and reliable description of the interactions between the TCR and pMHC molecules when including entropic terms.
(Tags: function of Anti-Mouse 2C TCR Monoclonal; function of Anti-Mouse 2C TCR Monoclonal antibody)