Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry

PA007331.m1: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Mouse IgG1 kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: 3G8.
Isotype:  Mouse IgG1 kappa.
Source: The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007331.h1Fs: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody.
Clone: 3G8.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007332.m1-b: Biotinylated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant  mouse IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Clone: 3G8.
Isotype:  Mouse IgG1 kappa.
Source: The site-directed biotinylated recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 3G8 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Syd Labs provides the following recombinant anti-human CD16 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Biotin Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
FITC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Pacific Blue Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 488 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 549 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 594 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 647 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 700 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP/Cy5.5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)

Background:

The recombinant 3G8 antibody binds to human and non-human primate CD16 or FcγRIII, a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 consists of Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16.

References of Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry:

Trade-offs shaping transmission of sylvatic dengue and Zika viruses in monkey hosts.
Hanley, K. A., et al. Nat Commun. 2024 Mar 27;15(1):2682. doi: 10.1038/s41467-024-46810-x. PMID: 38538621
Cells were subsequently stained with mouse anti-human CD20 (clone 2H7, Alexa-700, catalog number 560631, lot number 0209371, 1:16 dilution), mouse anti-human CD3 (clone SP34-2, Alexa-700, catalog number catalog number 557917, lot number 9185577, 1:16 dilution), mouse anti-human CD14 (clone M5E2, Alexa-700, catalog number 557923, lot number 0023216, 1:8 dilution), mouse anti-human CD16 (clone 3G8, PE, catalog number 556619, lot number 9107538, 1:18 dilution) (all antibodies purchased from BD Biosciences and used at the manufacturer recommended dilution), and Live/Dead Fixable Blue (Invitrogen, Waltham, MA, USA) in a volume of 100 μL for 1 hour in the dark with gentle agitation every 15 minutes. Subsequently, erythrocytes were lysed with BD FACS Lysing Solution (BD Biosciences) diluted in molecular biology grade water (Corning, Corning, NY, USA) for 12 min followed by immediate centrifugation at 500 g for 5 minutes at room temperature. ...Mosquito-borne dengue (DENV) and Zika (ZIKV) viruses originated in Old World sylvatic (forest) cycles involving monkeys and canopy-living Aedes mosquitoes. ...Studies of the trade-offs that shape within-host dynamics and transmission of these viruses are lacking, hampering efforts to predict spillover and spillback. ...In both hosts, ZIKV reached high titers that translated into high transmission to mosquitoes; in contrast DENV-2 replicated to low levels and, unexpectedly, transmission occurred only when serum viremia was below or near the limit of detection. ...Spillover of zoonotic, arthropod-borne viruses (arboviruses) into humans is accelerating, with outcomes ranging from dead-end infections to pandemics.
Tags: anti-human CD16 3G8 in cancer research; anti-human CD16 3G8 mAb in cancer research

For more references about Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2) for flow cytometry