BP004746: Human RANKL/TNFSF11/CD254 Protein with No Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A, hFc tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP004797: Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the N-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.