BP005179: Human IL-12B/p40/NKSF2 Protein with the C-His Tag
Source: The recombinant Human IL-12B/p40/NKSF2 Protein, (amino acids Ile23-Ser328) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-12B protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-IL-12 Antibody protein with the C-hFc tag with the EC50 of 18.5 ng/ml determined by ELISA. The affinity constant of 98.1 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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