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Human IL-12B Protein

IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS

Catalog No. Product Name Size List Price (US$) Quantity
BP005179 Human IL-12B/p40/NKSF2 Protein with the C-His Tag 100UG 270.00
BP005179 Human IL-12B/p40/NKSF2 Protein with the C-His Tag 500UG 900.00
Description

BP005179: Human IL-12B/p40/NKSF2 Protein with the C-His Tag

Source: The recombinant Human IL-12B/p40/NKSF2 Protein, (amino acids Ile23-Ser328) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human IL-12B/p40/NKSF2 protein with the His tag at the C-terminus has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-12B protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-IL-12 Antibody protein with the C-hFc tag with the EC50 of 18.5 ng/ml determined by ELISA. The affinity constant of 98.1 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-12B/p40/NKSF2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human IL-12B/p40/NKSF2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following IL-12 Protein :
Biotinylated Human IL-12 Protein
Cynomolgus IL-12 Protein
Human IL-12 Protein
IL-12 Protein
Mouse IL-12 Protein
Human IL-12 R beta 1 Protein
Cynomolgus IL-12B Protein
Human IL-12B Protein

Syd Labs provides the following IL-12 ELISA Kit :
Human IL-12 ELISA Kit
Mouse IL-12 ELISA Kit

Syd Labs provides the following IL-12 Antibody :
IL-12 Antibody
IL-12B Antibody
IL-12p75 Antibody

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